{"title":"Bioinformatics Analysis of Oxidative Stress-Related Genes and Immune Infiltration Patterns in Vitiligo.","authors":"Mingmei Yang, Huiying Wang, Ruzhi Zhang","doi":"10.2147/CCID.S496781","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Vitiligo is an autoimmune disorder characterized by pigment loss, and current treatment options remain inadequate.</p><p><strong>Objective: </strong>This study aims to identify oxidative stress-related biomarkers and hub genes associated with vitiligo diagnosis through genomic analysis and to examine the role of immune cell infiltration in the pathogenesis of vitiligo.</p><p><strong>Methods: </strong>The mRNA expression profile dataset GSE75819 was retrieved from the GEO database. Differential expression of oxidative stress-related genes in vitiligo was analyzed using R software. Protein-protein interaction (PPI) analysis, gene ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted on the differentially expressed genes (DEGs). Immune cell infiltration between vitiligo and normal control groups was assessed using the CIBERSORT algorithm. Additionally, two machine learning algorithms were employed to identify hub genes, perform enrichment analyses, and evaluate their correlation with immune infiltration.</p><p><strong>Results: </strong>A total of 415 Oxidative Stress-DEGs were identified in vitiligo, including 317 up-regulated and 98 down-regulated genes. PPI analysis highlighted the significance of certain ribosomal protein genes. KEGG enrichment analysis suggested an association between vitiligo and various neurodegenerative conditions, particularly through pathways such as oxidative phosphorylation and ribosome biogenesis. GO enrichment analysis indicated that the hub genes were significantly enriched in mitochondrial-related activities. Significant differences in immune infiltration patterns were observed between vitiligo patients and normal controls. Machine learning algorithms identified oxidative stress-related key genes associated with vitiligo, notably the DCT gene, whose expression was strongly linked to the activity of specific immune cell subsets and melanin biosynthetic pathways.</p><p><strong>Conclusion: </strong>Oxidative stress-related DEGs, ribosomal proteins, immune infiltration, and hub genes related to melanin biosynthesis, particularly DCT, are closely associated with the pathogenesis of vitiligo. These findings enhance our understanding of vitiligo and may aid in identifying therapeutic targets for the disease.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"18 ","pages":"475-489"},"PeriodicalIF":1.9000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880687/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical, Cosmetic and Investigational Dermatology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/CCID.S496781","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Vitiligo is an autoimmune disorder characterized by pigment loss, and current treatment options remain inadequate.
Objective: This study aims to identify oxidative stress-related biomarkers and hub genes associated with vitiligo diagnosis through genomic analysis and to examine the role of immune cell infiltration in the pathogenesis of vitiligo.
Methods: The mRNA expression profile dataset GSE75819 was retrieved from the GEO database. Differential expression of oxidative stress-related genes in vitiligo was analyzed using R software. Protein-protein interaction (PPI) analysis, gene ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted on the differentially expressed genes (DEGs). Immune cell infiltration between vitiligo and normal control groups was assessed using the CIBERSORT algorithm. Additionally, two machine learning algorithms were employed to identify hub genes, perform enrichment analyses, and evaluate their correlation with immune infiltration.
Results: A total of 415 Oxidative Stress-DEGs were identified in vitiligo, including 317 up-regulated and 98 down-regulated genes. PPI analysis highlighted the significance of certain ribosomal protein genes. KEGG enrichment analysis suggested an association between vitiligo and various neurodegenerative conditions, particularly through pathways such as oxidative phosphorylation and ribosome biogenesis. GO enrichment analysis indicated that the hub genes were significantly enriched in mitochondrial-related activities. Significant differences in immune infiltration patterns were observed between vitiligo patients and normal controls. Machine learning algorithms identified oxidative stress-related key genes associated with vitiligo, notably the DCT gene, whose expression was strongly linked to the activity of specific immune cell subsets and melanin biosynthetic pathways.
Conclusion: Oxidative stress-related DEGs, ribosomal proteins, immune infiltration, and hub genes related to melanin biosynthesis, particularly DCT, are closely associated with the pathogenesis of vitiligo. These findings enhance our understanding of vitiligo and may aid in identifying therapeutic targets for the disease.
期刊介绍：
Clinical, Cosmetic and Investigational Dermatology is an international, peer-reviewed, open access journal that focuses on the latest clinical and experimental research in all aspects of skin disease and cosmetic interventions. Normal and pathological processes in skin development and aging, their modification and treatment, as well as basic research into histology of dermal and dermal structures that provide clinical insights and potential treatment options are key topics for the journal.
Patient satisfaction, preference, quality of life, compliance, persistence and their role in developing new management options to optimize outcomes for target conditions constitute major areas of interest.
The journal is characterized by the rapid reporting of clinical studies, reviews and original research in skin research and skin care.
All areas of dermatology will be covered; contributions will be welcomed from all clinicians and basic science researchers globally.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
