Initial motility and vitality predict the semen quality after long-term cryostorage, even in patients with restricted ejaculate parameters.

IF 3.2 2区 医学 Q1 ANDROLOGY
Andrology Pub Date : 2025-03-06 DOI:10.1111/andr.70019
Simone Bier, Daniela Hanke, Michael Zitzmann, Sabine Kliesch, Verena Nordhoff
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引用次数: 0

Abstract

Background: Cryopreservation of human semen is the cornerstone for preserving male fertility before gonadotoxic therapy or in cases of high variability in semen parameters. This is particular crucial in cases of severe oligoasthenoteratozoospermia (OAT), where diminished sperm counts may compromise planned intracytoplasmic sperm injection (ICSI) procedures. Previous investigations in donor programs have shown long-term storage effects, such as decreased motility in cryopreserved semen samples. However, these studies were based on patients exhibiting normozoospermic semen samples. To date, there has been no comprehensive evaluation of the effect of long-term cryostorage on sperm samples from individuals with compromised semen parameters.

Objectives: The aim of this study was to identify the effect of long-term cryostorage on semen parameters such as motility and vitality. Additionally, we sought to identify variables, which could aid in predicting motility and vitality following the freeze-thaw process.

Patients and methods: Within our center, we have archived sperm samples from 6022 patients cryopreserved between 2001 and 2019. Among these, 293 patients donated their samples for subsequent research following depot termination. We examined semen concentration, motility, morphology, and vitality of spermatozoa thawed after varying storage durations, alongside baseline metrics documented at the time of cryopreservation. Samples were stratified into three cohorts based on storage duration: 2.5 to ≤5 years, > 5 to ≤14 years, and > 14 years.

Results: Our analysis revealed no changes in motility (p = 0.44), vitality (p = 0.08), or morphology (p = 0.44) across the cohorts. Regression analysis demonstrated that initial motility and sperm concentration were significantly associated with post-cryostorage motility, whereas storage duration was not (p = 0.72). Similarly, there was no association between storage duration and post-thaw value 2 vitality (p = 0.64).

Discussion: The initial semen analysis as well as the evaluation of a short-term frozen sample immediately after cryopreservation, appeared to be the most important markers for predicting post-thaw motility and vitality.

Conclusion: Our results demonstrate the reliability of long-term cryostorage of human spermatozoa for fertility preservation, even in individuals with constrained semen quality at the time of cryopreservation.

初始运动和活力预测长期冷冻后的精液质量,即使对射精参数受限的患者也是如此。
背景:人类精液的冷冻保存是在促性腺毒素治疗前或在精液参数高度变异的情况下保存男性生育能力的基础。这在严重少弱异卵精子症(OAT)的病例中尤为重要,在这种情况下,精子数量减少可能会影响计划的卵胞浆内单精子注射(ICSI)手术。先前对捐赠者项目的调查显示了长期储存的影响,例如冷冻保存的精液样本的活力下降。然而,这些研究是基于显示正常精子的患者精液样本。迄今为止,还没有关于长期冷冻对精液参数受损个体精子样本影响的综合评估。目的:本研究的目的是确定长期冷冻保存对精液运动和活力等参数的影响。此外,我们试图确定变量,这有助于预测冻融过程后的运动性和活力。患者和方法:在我们的中心,我们已经存档了2001年至2019年期间冷冻保存的6022名患者的精子样本。其中293例患者在库终止后捐献样本供后续研究使用。我们检测了不同储存时间后解冻精子的精液浓度、活力、形态和活力,以及冷冻保存时记录的基线指标。根据储存时间将样本分为三个队列:2.5至≤5年,> 5至≤14年,> 14年。结果:我们的分析显示,在整个队列中,运动性(p = 0.44)、活力(p = 0.08)或形态学(p = 0.44)没有变化。回归分析显示,初始运动能力和精子浓度与冷冻后运动能力显著相关,而储存时间与冷冻后运动能力无关(p = 0.72)。同样,贮藏时间与解冻后活力值2也无相关性(p = 0.64)。讨论:最初的精液分析以及冷冻保存后立即短期冷冻样本的评估,似乎是预测解冻后运动和活力的最重要标志。结论:我们的研究结果证明了长期冷冻保存人类精子以保存生育能力的可靠性,即使在冷冻保存时精液质量受限的个体中也是如此。
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来源期刊
Andrology
Andrology ANDROLOGY-
CiteScore
9.10
自引率
6.70%
发文量
200
期刊介绍: Andrology is the study of the male reproductive system and other male gender related health issues. Andrology deals with basic and clinical aspects of the male reproductive system (gonads, endocrine and accessory organs) in all species, including the diagnosis and treatment of medical problems associated with sexual development, infertility, sexual dysfunction, sex hormone action and other urological problems. In medicine, Andrology as a specialty is a recent development, as it had previously been considered a subspecialty of urology or endocrinology
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