Differentiating between normal and inflammatory blood serum samples using spectrochemical analytical techniques and chemometrics.

Abstract

Inflammation detection in blood serum samples is commonly performed using clinical analyzers, which are expensive and complex and require specific labels or markers. Spectrochemical analytical techniques, such as laser-induced breakdown spectroscopy (LIBS) and laser-induced fluorescence (LIF), have emerged as alternative methods for qualitative and non-destructive analysis in various fields. This study explores applying LIBS and LIF techniques for label-free discrimination between normal and inflammatory blood serum samples. In the LIBS analysis, the serum samples are deposited on ashless filter paper and exposed to a high-power Nd:YAG laser source to induce plasma emission. The emitted light is dispersed in a spectrometer and an ICCD camera that captures the spectral lines. The LIF technique utilizes a diode-pumped solid-state laser source to excite the blood serum sample placed in a quartz cuvette. The resulting emission spectra are collected and analyzed using a spectrometer equipped with a CCD detector. The obtained spectroscopic data from both techniques is subjected to principal component analysis (PCA) and graph theory for classification and clustering. The PCA classified the two classes with a data variance of 85.4% and 92.8% based on the first two principal components (PCs) for LIBS and LIF spectra. The graph theory clustered the two classes with an accuracy of 76% and 100% based on LIBS and LIF spectra. The statistical methods effectively discriminate between normal and inflammatory serum samples, providing satisfactory results. The proposed spectrochemical methods offer several advantages over traditional clinical analyzers. They are cost-effective and rapid, making them suitable for the fast and reliable identification of serum samples in laboratories. The non-destructive nature of these techniques eliminates the need for specific labels or markers, further streamlining the analysis process.