Hannah Arnade, Irene E Ivie, Julie Gordon, John F Peroni
{"title":"Three-dimensional tissue culture supports the structure and function of equine synovial explants over 4 days.","authors":"Hannah Arnade, Irene E Ivie, Julie Gordon, John F Peroni","doi":"10.2460/ajvr.24.11.0357","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the feasibility of short-term 3-D tissue culture in maintaining the structure and function of equine synovial explants and demonstrate that day 4 explant characteristics were not significantly different from day 0. We hypothesized that a 4-day culture period in 3-D explant culture would not significantly disrupt synovial tissue health and function compared to baseline measurements.</p><p><strong>Methods: </strong>Synovial explants (n = 24/horse) from healthy carpal joints of 5 horses were cultured in 12-well plates using tissue-stabilizing agar rings. Explants were evaluated in triplicate or quadruplicate at days 0, 2, and 4 for metabolism, viability, histologic parameters, cytokine secretion, and hyaluronic acid production.</p><p><strong>Results: </strong>No tested parameters showed significant changes by day 4. Average cell viability was 87.99% (95% CId4-d0, -4.6% to 12.99%), and histologic grading showed slight but nonsignificant changes. Mitochondrial NADH production from pyruvate and malate on day 4 remained at 85% to 90% that of day 0 (Cohen d, 0.184, 0.153). Hyaluronic acid production over days 2 to 4 was 95.98% that of days 0 to 2 (95% CIday4/day2, 73.82% to 118.14%). Cytokine secretion showed nonsignificant decreases (TNFαday4/day2, 0.518; CI, 0.159 to 0.417; IL-1βday4/day2, 0.520; CI, 0.150 to 0.393). Explants exhibited macrophagic character by staining diffusely with anti-CD11b and CD14 antibodies.</p><p><strong>Conclusions: </strong>Our culture system repeatably maintained the structural and functional integrity of equine synovial explants over 4 days.</p><p><strong>Clinical relevance: </strong>This study establishes a standardized method for culturing equine synovial explants to the aim of accurately modeling of joint diseases and the effects of articular therapeutics.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-8"},"PeriodicalIF":1.3000,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2460/ajvr.24.11.0357","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To evaluate the feasibility of short-term 3-D tissue culture in maintaining the structure and function of equine synovial explants and demonstrate that day 4 explant characteristics were not significantly different from day 0. We hypothesized that a 4-day culture period in 3-D explant culture would not significantly disrupt synovial tissue health and function compared to baseline measurements.
Methods: Synovial explants (n = 24/horse) from healthy carpal joints of 5 horses were cultured in 12-well plates using tissue-stabilizing agar rings. Explants were evaluated in triplicate or quadruplicate at days 0, 2, and 4 for metabolism, viability, histologic parameters, cytokine secretion, and hyaluronic acid production.
Results: No tested parameters showed significant changes by day 4. Average cell viability was 87.99% (95% CId4-d0, -4.6% to 12.99%), and histologic grading showed slight but nonsignificant changes. Mitochondrial NADH production from pyruvate and malate on day 4 remained at 85% to 90% that of day 0 (Cohen d, 0.184, 0.153). Hyaluronic acid production over days 2 to 4 was 95.98% that of days 0 to 2 (95% CIday4/day2, 73.82% to 118.14%). Cytokine secretion showed nonsignificant decreases (TNFαday4/day2, 0.518; CI, 0.159 to 0.417; IL-1βday4/day2, 0.520; CI, 0.150 to 0.393). Explants exhibited macrophagic character by staining diffusely with anti-CD11b and CD14 antibodies.
Conclusions: Our culture system repeatably maintained the structural and functional integrity of equine synovial explants over 4 days.
Clinical relevance: This study establishes a standardized method for culturing equine synovial explants to the aim of accurately modeling of joint diseases and the effects of articular therapeutics.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
