Ex vivo coccidioidal antigen chitinase-1 stimulation increases production of interleukin-1β in healthy cats.

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

American journal of veterinary research Pub Date : 2025-03-05 DOI:10.2460/ajvr.24.11.0332

Danielle Gordon, Jared A Jaffey, Lisa F Shubitz, Michael D L Johnson, Charlotte Bolch, Sanjay V Menghani, Brooke Benecke

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引用次数: 0

Abstract

Objective: To evaluate coccidioidal antigen-stimulated production of cytokines in healthy cats and determine optimum testing conditions (ie, incubation time and antigen concentration).

Methods: 6 client-owned, healthy cats that were seronegative for Coccidioides spp antibodies were included in this prospective experimental study. Whole blood cultures were performed with exposure to PBS (vehicle control) or a coccidioidal antigen (recombinant coccidioidal antigen chitinase-1 [rCTS1]105-310) at 5 final well concentrations (1 µg/mL, 10 µg/mL, 25 µg/mL, 50 µg/mL, and 75 µg/mL) with incubation times of 12 hours and 24 hours. A validated feline-specific, multiplex bead-based assay was used to measure 10 cytokines in cell culture supernatant.

Results: Supernatant concentrations of IL-1β increased when stimulated with rCTS1105-310 for 12 hours at 50 µg/mL (274.4 pg/mL; P = .02) and 75 µg/mL (298.4 pg/mL; P = .003) compared to control (PBS; 102.9 pg/mL). Similarly, IL-1β concentrations increased after exposure to rCTS1105-310 for 24 hours at 50 µg/mL (340.3 pg/mL) and 75 µg/mL (364.8 pg/mL) compared to control (110.5 pg/mL; P = .04). Production of IL-1β was greater when whole blood was stimulated with rCTS1105-310 at 1 µg/mL for 24 hours (237.0 pg/mL) compared with 12 hours (116.8 pg/mL; P = .04). No other differences in cytokine concentrations were identified at either incubation time or rCTS1105-310 concentration compared to control.

Conclusions: Exposure of whole blood to rCTS1105-310 increases production of IL1β in healthy cats. Incubation time and rCTS1105-310 concentration did not seem to impact production of most cytokines in this study.

Clinical relevance: These results provide information on rCTS1105-310-stimulated cytokine concentrations in healthy cats.

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体外球虫抗原几丁质酶-1刺激增加健康猫白细胞介素-1β的产生。

目的：评价健康猫球虫抗原刺激细胞因子的产生，确定最佳试验条件（即孵育时间和抗原浓度）。方法：6只客户拥有的球虫抗体血清阴性的健康猫被纳入这项前瞻性实验研究。全血培养以5个终孔浓度（1µg/mL, 10µg/mL, 25µg/mL, 50µg/mL, 75µg/mL）暴露于PBS（载体对照）或球虫抗原（重组球虫抗原几丁质酶-1 [rCTS1]105-310），孵育时间为12小时和24小时。一种经过验证的猫特异性的、基于多重头的检测方法用于测量细胞培养上清中的10种细胞因子。结果：rCTS1105-310以50µg/mL (274.4 pg/mL；P = 0.02)和75µg/mL (298.4 pg/mL；P = 0.003)，与对照组(PBS；102.9 pg / mL)。同样，暴露于rCTS1105-310中，与对照组(110.5 pg/mL；P = .04)。以1µg/mL浓度rCTS1105-310刺激全血24小时（237.0 pg/mL）比12小时(116.8 pg/mL；P = .04)。与对照组相比，在孵育时间或rCTS1105-310浓度下，细胞因子浓度均未发现其他差异。结论：全血暴露于rCTS1105-310可增加健康猫il - 1β的产生。在本研究中，孵育时间和rCTS1105-310浓度似乎不影响大多数细胞因子的产生。临床相关性：这些结果提供了rcts1105 -310刺激的健康猫体内细胞因子浓度的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.