Stable plastid transformation in kiwifruit (Actinidia chinensis)

IF 4.6 4区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

aBIOTECH Pub Date : 2024-11-28 DOI:10.1007/s42994-024-00186-0

Qiqi Chen, Yuyong Wu, Yanchang Wang, Jiang Zhang, Shengchun Li

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引用次数: 0

Abstract

Plastid transformation offers valuable benefits in plant biotechnology, such as high-level transgene expression and the absence of gene silencing. Here we describe the first protocol of a plastid transformation system for a woody vine (liana) kiwifruit (Actinidia chinensis). The transgenic DNA carries a spectinomycin-resistance gene (aadA) cassette and a green fluorescent protein (GFP) reporter gene cassette, flanked by two adjacent kiwifruit plastid genome sequences, thereby allowing targeted insertion between the trnfM and trnG genes. Six spectinomycin-resistant shoots were obtained out of 12 plates subjected to bombardment, and two were positive events, confirmed through PCR and Southern blot analyses. The GFP was localized to plastids as monitored by confocal laser scanning microscopy and reached 2.5% of leaf total soluble protein. Success in kiwifruit extends transplastomic technology of woody species beyond poplar, and will provide an attractive biosynthetic chassis for molecular farming.

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来源期刊

aBIOTECH

CiteScore

7.70

自引率

2.80%

发文量