In vitro inhibitory activity of indole alkaloid derivatives against porcine epidemic diarrhea virus

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-03-07 DOI:10.1007/s00705-025-06251-3

Yaoying Jian, Siqi Dong, Qianyi Zhang, Jiali Pan, Ruiming Hu, Zhen Ding, Huansheng Wu, Shaoyong Ke, Zheng Chen

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Abstract

Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus that can cause acute diarrhea, vomiting, dehydration, and high mortality of newborn piglets, leading to huge economic losses to the world pig industry. Given the limited efficacy of current PEDV vaccines, there is an urgent need for the development of antiviral drugs. In this study, the antiviral effects of 17 synthesized indole alkaloid derivatives against PEDV were investigated. It was observed that indole alkaloid derivative no. 14 exhibited significant inhibition of PEDV replication in a dose-dependent manner. Furthermore, time-of-addition assays and quantitative real-time PCR (QPCR) showed that delayed administration of this compound resulted in a weaker inhibitory effect on PEDV compared to early treatment. Mechanistic analysis revealed that this compound exerts its inhibitory effects during the entry stage of the PEDV life cycle. This study demonstrates the anti-PEDV effects of indole alkaloid derivative no. 14, suggesting its potential as a candidate drug for treating PEDV infections.

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吲哚类生物碱衍生物对猪流行性腹泻病毒的体外抑制活性研究

猪流行性腹泻病毒（PEDV）是一种肠道冠状病毒，可引起新生仔猪急性腹泻、呕吐、脱水和高死亡率，给世界养猪业造成巨大的经济损失。鉴于目前PEDV疫苗的疗效有限，迫切需要开发抗病毒药物。本研究研究了17种合成的吲哚类生物碱衍生物对PEDV的抗病毒作用。观察到吲哚生物碱衍生物no。其中14种以剂量依赖的方式对PEDV复制表现出显著的抑制作用。此外，添加时间测定和实时荧光定量PCR （QPCR）显示，与早期治疗相比，延迟给药导致该化合物对PEDV的抑制作用较弱。机制分析表明，该化合物在PEDV生命周期的进入阶段发挥抑制作用。本研究证实了吲哚生物碱衍生物no的抗pedv作用。14，提示其作为治疗PEDV感染的候选药物的潜力。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.