Molecular characterization of a polymycovirus in Leptosphaeria biglobosa

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-03-06 DOI:10.1007/s00705-025-06253-1

Nesma M. Samir, Jacob Locke-Gotel, Syun-Ichi Urayama, Adel A. El-Morsi, Ghada A. El-Sherbeny, Yongju Huang, Bruce D. L. Fitt, Hiromitsu Moriyama, Robert H. A. Coutts, Ioly Kotta-Loizou

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引用次数: 0

Abstract

Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome has five dsRNA segments, ranging in length from 1,180 bp to 2,402 bp. Each dsRNA has a single open reading frame flanked by 5′ and 3′ untranslated regions. The proteins encoded by dsRNAs 1 and 3, an RNA-dependent RNA polymerase (RdRP) and a methyltransferase, respectively, have significant similarity to those of Plasmopara viticola lesion associated polymycovirus 1. The proline-alanine-serine-rich protein encoded by dsRNA 5 is similar to that of Erysiphe necator associated polymycovirus 1. The proteins encoded by dsRNAs 2 and 4 have significant similarity to those of a mycovirus identified in Alternaria sp. FA0703. Phylogenetic analysis based on RdRP sequences showed that this virus clusters with members of the family Polymycoviridae. Based on these observations, this virus, which we have named "Leptosphaeria biglobosa polymycovirus 1", should be classified as a member of the family Polymycoviridae. This is the first report of a polymycovirus in L. biglobosa.

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一种细钩绦虫多分枝病毒的分子特性研究

大叶细球绦虫是甘蓝型油菜的一种植物致病性子囊菌，可引起瘤茎溃烂/黑腿。从该真菌中分离出一种新的双链RNA （dsRNA）分枝病毒。病毒基因组有5个dsRNA片段，长度从1180 bp到2402bp不等。每个dsRNA有一个单独的开放阅读框，两侧是5 ‘和3 ’未翻译区域。分别由RNA依赖的RNA聚合酶（RdRP）和甲基转移酶dsRNAs 1和dsRNAs 3编码的蛋白与葡萄浆原病变相关的多分枝病毒1具有显著的相似性。dsRNA 5编码的富含脯氨酸-丙氨酸-丝氨酸的蛋白与赤毒相关多分枝病毒1相似。dsRNAs 2和dsRNAs 4编码的蛋白与在Alternaria sp. FA0703中鉴定的分枝病毒具有显著的相似性。基于RdRP序列的系统发育分析表明，该病毒属于多分枝病毒科成员。基于这些观察结果，我们将该病毒命名为“大叶钩端绦虫多分枝病毒1号”，应归类为多分枝病毒科成员。这是首次报道的一种多分枝病毒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.