Biosynthesis of gastrodin via multi-module UDPG supply and site-directed mutagensis of glycosyltransferase in Escherichia coli

IF 3.9 2区化学 Q2 CHEMISTRY, PHYSICAL

Molecular Catalysis Pub Date : 2025-03-07 DOI:10.1016/j.mcat.2025.114953

Zhao Zhang , Xinying Wang , Xin Sha , Guohao Qiao , Fengyi Niu , Hong Ming , Caixia Cui

{"title":"Biosynthesis of gastrodin via multi-module UDPG supply and site-directed mutagensis of glycosyltransferase in Escherichia coli","authors":"Zhao Zhang , Xinying Wang , Xin Sha , Guohao Qiao , Fengyi Niu , Hong Ming , Caixia Cui","doi":"10.1016/j.mcat.2025.114953","DOIUrl":null,"url":null,"abstract":"<div><div>Gastrodin, the primary bioactive compound in <em>Gastrodia elata</em>, has therapeutic potential for nervous system disorders. However, its low yield limits the industrial-scale production. Microbial synthesis faces two major challenges: restricted UDP-glucose (UDPG) availability and inefficient glycosyltransferase activity. This study addressed these limitations by engineering a multi-module UDPG biosynthetic pathway and enhancing glycosyltransferase efficiency through site-directed mutagenesis. The recombinant strain G19, including the susy, ppk, pgm, and galU genes for UDPG production, achieved increased gastrodin yield 51-fold. In addition, the single-point mutant A347 V improved glycosyltransferase activity by over five-fold. The advanced strain G20 further improved production, reaching 4.56 g/L in shake-flask cultures. This study establishes a robust approach for glycoside production through optimized UDPG biosynthesis and enzyme engineering.</div></div>","PeriodicalId":393,"journal":{"name":"Molecular Catalysis","volume":"578 ","pages":"Article 114953"},"PeriodicalIF":3.9000,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Catalysis","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468823125001397","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Gastrodin, the primary bioactive compound in Gastrodia elata, has therapeutic potential for nervous system disorders. However, its low yield limits the industrial-scale production. Microbial synthesis faces two major challenges: restricted UDP-glucose (UDPG) availability and inefficient glycosyltransferase activity. This study addressed these limitations by engineering a multi-module UDPG biosynthetic pathway and enhancing glycosyltransferase efficiency through site-directed mutagenesis. The recombinant strain G19, including the susy, ppk, pgm, and galU genes for UDPG production, achieved increased gastrodin yield 51-fold. In addition, the single-point mutant A347 V improved glycosyltransferase activity by over five-fold. The advanced strain G20 further improved production, reaching 4.56 g/L in shake-flask cultures. This study establishes a robust approach for glycoside production through optimized UDPG biosynthesis and enzyme engineering.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular Catalysis Chemical Engineering-Process Chemistry and Technology

CiteScore

6.90

自引率

10.90%

发文量

700

审稿时长

40 days

期刊介绍： Molecular Catalysis publishes full papers that are original, rigorous, and scholarly contributions examining the molecular and atomic aspects of catalytic activation and reaction mechanisms. The fields covered are: Heterogeneous catalysis including immobilized molecular catalysts Homogeneous catalysis including organocatalysis, organometallic catalysis and biocatalysis Photo- and electrochemistry Theoretical aspects of catalysis analyzed by computational methods