Phthalocyanine based metal organic frameworks for electrochemical detection of human epidermal growth factor receptor 2

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-03-03 DOI:10.1016/j.bioelechem.2025.108966

Lunathi Ncwane , Philani Mashazi , Tebello Nyokong

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引用次数: 0

Abstract

Herein, we report on the development of a phthalocynaine based metal organic frameworks (MOF) for the detection of human epidermal growth factor receptor 2 (HER2). Phthalocyanines (Pcs) exhibit good redox properties, hence their utilization as precursors for the synthesis of Pc based MOFs. The successful preparation of the MOF was confirmed using X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and Brunner Emmet Teller (BET) analysis. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were utilized for electrochemical characterization of the Co octacarboxy Pc (CoOCPc), and Co-CoOCPc-MOF modified glassy carbon electrode (GCE). Differential pulse votammetry was employed for detection of HER2, which is a biomarker for cancer. The selectivity towards HER2 biomarker was accomplished by attaching an aptamer (Apt) onto the MOF modified glassy carbon surface. The GCE/Co-CoOCPc-MOF/Nf/Apt (Nf = Nafion) showed excellent analytical parameters with lowest limit of detection of 5.4 × 10 ng/mL, good repeatability and stability.

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.