Genetic diversity analysis of benzimidazole resistance-associated genes in Haemonchus contortus from four regions in southern Xinjiang

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-02-28 DOI:10.1016/j.vetpar.2025.110426

Reyilanmu Tuerhong , Waresi Tuersong , Ailixire Maimaiti , Hailiqiemu Maimaitiyiming , Ying Zhang , Dilare Xuekelaiti , Abudusaimaiti Tuoheti , Lianxi Xin , Saifuding Abula

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引用次数: 0

Abstract

To assess the genetic diversity of the I β-tubulin haplotype gene associated with benzimidazoles (BZs) resistance in Haemonchus contortus populations across southern Xinjiang, the sampled populations from four distinct areas to detect the BZs resistance-related I-β tubulin gene and analyse its genetic sequences and structure. The results revealed substantial haplotype diversity and nucleotide polymorphisms across the four populations, with nucleotide diversity (Pi) ranging from 0.03471 to 0.10390 and haplotype diversity (Hd) from 0.945 to 1.000. A low genetic differentiation index (Fst) among populations in the four regions ranging from 0.03354 to 0.12056 suggests weak genetic structure differentiation and frequent gene flow between populations. AMOVA indicated that 91 % of genetic variation was due to within-population differences, suggesting minimal impact of geographical location on H. contortus population structure in southern Xinjiang. Phylogenetic and network analyses showed that 36 H. contortus haplotypes from diverse geographic sources clustered into a single branch with interwoven patterns. Notably, drug resistance mutations showed two origins at E198A, while multiple origins likely exist for the F200Y mutation. This study provides the first genetic diversity analysis of benzimidazole resistance-related genes in H. contortus populations in southern Xinjiang, offering preliminary insights into the spread of resistance and supporting disease control efforts.

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.