Genetic diversity analysis of benzimidazole resistance-associated genes in Haemonchus contortus from four regions in southern Xinjiang

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-02-28 DOI:10.1016/j.vetpar.2025.110426

Reyilanmu Tuerhong , Waresi Tuersong , Ailixire Maimaiti , Hailiqiemu Maimaitiyiming , Ying Zhang , Dilare Xuekelaiti , Abudusaimaiti Tuoheti , Lianxi Xin , Saifuding Abula

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Abstract

To assess the genetic diversity of the I β-tubulin haplotype gene associated with benzimidazoles (BZs) resistance in Haemonchus contortus populations across southern Xinjiang, the sampled populations from four distinct areas to detect the BZs resistance-related I-β tubulin gene and analyse its genetic sequences and structure. The results revealed substantial haplotype diversity and nucleotide polymorphisms across the four populations, with nucleotide diversity (Pi) ranging from 0.03471 to 0.10390 and haplotype diversity (Hd) from 0.945 to 1.000. A low genetic differentiation index (Fst) among populations in the four regions ranging from 0.03354 to 0.12056 suggests weak genetic structure differentiation and frequent gene flow between populations. AMOVA indicated that 91 % of genetic variation was due to within-population differences, suggesting minimal impact of geographical location on H. contortus population structure in southern Xinjiang. Phylogenetic and network analyses showed that 36 H. contortus haplotypes from diverse geographic sources clustered into a single branch with interwoven patterns. Notably, drug resistance mutations showed two origins at E198A, while multiple origins likely exist for the F200Y mutation. This study provides the first genetic diversity analysis of benzimidazole resistance-related genes in H. contortus populations in southern Xinjiang, offering preliminary insights into the spread of resistance and supporting disease control efforts.

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南疆4个地区弯曲血蜱苯并咪唑抗性相关基因遗传多样性分析

为评估南新疆弯血蜱种群对苯并咪唑（BZs）抗性相关的I-β微管蛋白单倍型基因的遗传多样性，对4个不同地区的弯血蜱种群进行了BZs抗性相关的I-β微管蛋白基因检测，并对其基因序列和结构进行了分析。结果表明，4个群体具有丰富的单倍型多样性和核苷酸多态性，核苷酸多样性（Pi）在0.03471 ~ 0.10390之间，单倍型多样性（Hd）在0.945 ~ 1.000之间。4个地区居群间遗传分化指数（Fst）较低，介于0.03354 ~ 0.12056之间，表明居群间遗传结构分化弱，基因流动频繁。AMOVA分析结果表明，91% %的遗传变异是由种群内差异引起的，表明地理位置对南疆弯毛鼠种群结构的影响很小。系统发育分析和网络分析表明，来自不同地理来源的36个弯毛鼠单倍型聚集在一起，形成一个交织的分支。值得注意的是，耐药突变在E198A显示两个起源，而F200Y突变可能存在多个起源。本研究首次对新疆南疆弯纹鼠种群苯并咪唑抗性相关基因进行了遗传多样性分析，为其抗性传播提供了初步认识，并为疾病控制工作提供了支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.