Antifungal spectrum of chitosan films added with lactic acid bacteria cell-free supernatants

Food and Humanity Pub Date : 2025-03-03 DOI:10.1016/j.foohum.2025.100552

Bryan López-Pérez, David Mejía-Reyes, Raymundo Rosas-Quijano, Lourdes Adriano-Anaya, Adriana Sánchez-Gutiérrez, Alfredo Vázquez-Ovando

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Abstract

Lactic acid bacteria (LAB) are considered GRAS, and some strains have antifungal activity, so they can be used to inhibit the growth of fungi associated with spoilage of fruits and vegetables. The aim of this work was to evaluate the antifungal effect of edible films made with chitosan (1.5 % w/v) added with CFS of LAB against the phytopathogenic fungi Colletotrichum gloeosporioides, Fusarium sp. and Penicillium sp. AT21H10. Due to their in vitro antifungal capacity, the CFS of LAB, Lactiplantibacillus plantarum TEP15, Lacticaseibacillus paracasei TEP8 and Lactiplantibacillus pentosus TEJ4, were first selected. Film-forming solutions were spiked with 100 %, 50 %, 25 %, 12.5 %, and 6.5 % (v/v) of CFS from each of the LAB and their ability to inhibit spore germination and mycelium growth was evaluated. The films were effective in inhibiting both spore germination and mycelium growth of the three phytopathogenic fungi. CFS concentrations lower than 50 % had greater antifungal activity. The average inhibition values were > 70 % against C. gloeosporioides, 90–100 % against Fusarium sp. and 60–80 % against Penicillium sp. AT21H10.

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乳酸菌无细胞上清添加壳聚糖膜的抗真菌谱

乳酸菌（LAB）被认为是GRAS，一些菌株具有抗真菌活性，因此它们可以用来抑制与水果和蔬菜腐败相关的真菌的生长。以壳聚糖（1.5 % w/v）为原料，添加CFS制备食用膜，考察其对植物病原菌炭疽菌（Colletotrichum gloeosporioides）、镰刀菌（Fusarium sp.）和青霉菌（Penicillium sp. AT21H10）的抑制作用。由于它们的体外抗真菌能力，我们首先选择了LAB的CFS，即植物乳杆菌TEP15、副干酪乳杆菌TEP8和丁香乳杆菌TEJ4。在成膜溶液中分别加入100 %、50 %、25 %、12.5 %和6.5 % （v/v）的CFS，并对其抑制孢子萌发和菌丝生长的能力进行评价。该膜对三种植物病原真菌的孢子萌发和菌丝生长均有抑制作用。CFS浓度低于50 %具有较强的抗真菌活性。对黄孢霉的平均抑制值为>； 70 %，对镰刀菌的平均抑制值为90-100 %，对青霉菌AT21H10的平均抑制值为60-80 %。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Food and Humanity

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