A portable ascorbic acid touch-in personal glucose meter based on enzyme inhibition cascade reaction for on-site rapid detection of organophosphorus pesticides

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-03-05 DOI:10.1016/j.snb.2025.137576

Jing Yang , Yaqin Zhang , Xingbo Shi, Yan Lv

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引用次数: 0

Abstract

Rapid and portable detection technology of organophosphorus pesticides (OPs) is urgently needed to ensure food and environmental safety. Herein, a simple and portable method for in-field detection of OPs was established based on an ascorbic acid (AA) touch-in personal blood glucose meter (PGM) triggered by the inhibition of alkaline phosphatase (ALP). Particularly, AA was first used as a bridge to construct a cascade strategy ALP-AA-[Fe(CN)₆]^3- for PGM readout, as it has been proven to trigger stronger PGM readings due to its strong reducibility and double electron generation. In the presence of OPs, the enzymatic activity of ALP was inhibited to produce less AA, resulting in a weakened reduction of [Fe(CN)₆]^3- on the test strip and a decrease of the electrochemical signal. The PGM analysis platform showed a good linear response at concentrations of 10–40 μg L⁻¹ of dimethoate, with a detection limit of 7.55 μg L⁻¹, and its practicability and reliability were verified by the recovery results in vegetables and fruits. With the advantages of simplicity and portability, this method can complete the entire field detection process in 20 min. Hence, this work provides a promising candidate method for the rapid on-site detection of OPs in food and the environment.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.