Genotyping of extended-spectrum beta-lactamase-producing Escherichia coli isolated from wastewater of dairy farms in East Java, Indonesia.

IF 1.7 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Veterinary World Pub Date : 2025-01-01 Epub Date: 2025-01-27 DOI:10.14202/vetworld.2025.162-171

Fidi Nur Aini Eka Puji Dameanti, Sheila Marty Yanestria, Mustofa Helmi Effendi, Hani Plumeriastuti, Wiwiek Tyasningsih, Emmanuel Nnabuike Ugbo, Rahayu Sutrisno, Muhammad Ali Akramsyah Safri

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Abstract

Background and aim: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in dairy farm wastewater represents a significant threat to environmental and public health due to the dissemination of antibiotic-resistance genes. This study investigates the molecular profiles of ESBL-producing E. coli isolates harboring bla _CTX-M, bla _TEM, and bla _SHV genes from dairy farm wastewater in East Java, Indonesia.

Materials and methods: Wastewater samples (n = 342) were collected from six major dairy regions in East Java: Pasuruan, Malang, Tulungagung, Blitar, Batu, and Kediri. The samples underwent bacterial isolation, identification, and ESBL phenotypic screening using the double-disk synergy test (DDST). Molecular genotyping of bla _CTX-M, bla _TEM, and bla _SHV was conducted using a polymerase chain reaction.

Results: Of the samples, 69.30% (237/342) tested positive for E. coli, with 32.91% (78/237) identified as ESBL producers through DDST. Molecular genotyping confirmed ESBL genes in 28.20% (22/78) of the isolates. The genotypic distribution was 77.2% (17/22) bla _TEM, 4.5% (1/22) bla _CTX-M, 9.0% (2/22) bla _TEM + bla _SHV, and 9.0% (2/22) bla _TEM + bla _CTX-M. These findings highlight the dominance of bla _TEM and the presence of multi-gene combinations in East Java's dairy farm wastewater.

Conclusion: The identified molecular profiles underscore dairy farm wastewater as a critical reservoir for antibiotic resistance genes, particularly bla _TEM. Addressing this issue necessitates stringent antimicrobial use policies, improved wastewater management, and enhanced biosecurity measures. These findings support a One Health approach to mitigate environmental and public health risks associated with AMR.

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来源期刊

Veterinary World Multiple-

CiteScore

3.60

自引率

12.50%

发文量

317

审稿时长

16 weeks

期刊介绍： Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.