Exosomes containing miR-148a-3p derived from mesenchymal stem cells suppress epithelial-mesenchymal transition in lens epithelial cells.

IF 5.4 2区 医学 Q1 CELL & TISSUE ENGINEERING
Jingyu Ma, Qihang Sun, Yijia Chen, Jinyan Li, Shuyi Chen, Lixia Luo
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Abstract

Epithelial-mesenchymal transition (EMT) of lens epithelial cells (LECs) is responsible for the development of fibrotic cataracts, which contribute to severe visual impairment. Recent evidence has shown that mesenchymal stem cell-derived exosomes (MSC-Exo) can attenuate EMT in several tissues. However, the effect of MSC-Exo on EMT in LECs (LECs-EMT) has not been determined. In this study, we isolated exosomes from human umbilical cord MSCs (hucMSC-Exo) and evaluated their effect on LECs-EMT both in vitro and in vivo. HucMSC-Exo application significantly suppressed the expression of mesenchymal cell-associated genes while increasing the expression of epithelial cell-associated genes. Cell proliferation and migration of LECs undergoing EMT were inhibited after hucMSC-Exo treatment. The volume of EMT plaques in mice with injury-induced anterior subcapsular cataract (ASC) was significantly reduced in the hucMSC-Exo-treated group. Furthermore, miR-148a-3p was abundant in hucMSC-Exo. After transfection with miR-148a-3p inhibitor, the anti-fibrotic effect of hucMSC-Exo was attenuated in LECs-EMT. A dual-luciferase reporter assay identified PRNP as a direct target gene of miR-148a-3p. Furthermore, we verified that hucMSC-Exo inhibited LECs-EMT through the miR-148a-3p/PRNP axis and the potential downstream ERK signaling pathway. Taken together, our work reveals the inhibitory effect of hucMSC-Exo on LECs-EMT and the underlying mechanism involved, which may provide potential therapeutic options for fibrotic cataracts.

来自间充质干细胞的含有miR-148a-3p的外泌体抑制晶状体上皮细胞的上皮-间充质转化。
晶状体上皮细胞(LECs)的上皮-间充质转化(EMT)与纤维化性白内障的发展有关,从而导致严重的视力损害。最近的证据表明,间充质干细胞衍生的外泌体(MSC-Exo)可以减弱几种组织中的EMT。然而,MSC-Exo对LECs EMT (LECs-EMT)的影响尚未确定。在这项研究中,我们从人脐带间充质干细胞中分离出外泌体(hucMSC-Exo),并在体外和体内评估了它们对LECs-EMT的影响。应用HucMSC-Exo显著抑制间充质细胞相关基因的表达,同时增加上皮细胞相关基因的表达。经EMT处理的LECs细胞增殖和迁移受到抑制。损伤性前囊下白内障(ASC)小鼠的EMT斑块体积在hucmsc - exo处理组显著减少。此外,miR-148a-3p在hucMSC-Exo中含量丰富。转染miR-148a-3p抑制剂后,在lec - emt中,hucMSC-Exo的抗纤维化作用减弱。双荧光素酶报告基因检测鉴定PRNP是miR-148a-3p的直接靶基因。此外,我们验证了hucMSC-Exo通过miR-148a-3p/PRNP轴和潜在的下游ERK信号通路抑制LECs-EMT。综上所述,我们的工作揭示了humsc - exo对LECs-EMT的抑制作用及其潜在机制,这可能为纤维化性白内障的治疗提供潜在的选择。
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来源期刊
Stem Cells Translational Medicine
Stem Cells Translational Medicine CELL & TISSUE ENGINEERING-
CiteScore
12.90
自引率
3.30%
发文量
140
审稿时长
6-12 weeks
期刊介绍: STEM CELLS Translational Medicine is a monthly, peer-reviewed, largely online, open access journal. STEM CELLS Translational Medicine works to advance the utilization of cells for clinical therapy. By bridging stem cell molecular and biological research and helping speed translations of emerging lab discoveries into clinical trials, STEM CELLS Translational Medicine will help move applications of these critical investigations closer to accepted best patient practices and ultimately improve outcomes. The journal encourages original research articles and concise reviews describing laboratory investigations of stem cells, including their characterization and manipulation, and the translation of their clinical aspects of from the bench to patient care. STEM CELLS Translational Medicine covers all aspects of translational cell studies, including bench research, first-in-human case studies, and relevant clinical trials.
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