Silicon modulates nitrogen and secondary metabolism in Glycyrrhiza uralensis under drought and salt stress conditions.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Guohui Chen, Xiaojia Zhang, Gaochang Cui, Wenjin Zhang, Qiuxian Bai, Xinhui Zhang
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Abstract

Glycyrrhiza uralensis Fisch (G. uralensis) is a key species for windbreak and sand fixation, possessing notable pharmacological and economic value. However, the yield of G. uralensis is considerably impacted due to its cultivation in arid, semi-arid, and salt-affected regions. Silicon (Si) has been reported to improve plant tolerance to drought and salt stress by regulating nitrogen and secondary metabolism. Herein, the effects of Si treatment on nitrogen and secondary metabolism of G. uralensis seedlings under drought (D), salt (S), and drought-salt (SD) stresses were investigated in combination with physiological and transcriptomic analyses. The results indicated that stress conditions significantly inhibited the growth of G. uralensis seedlings by suppressing nitrogen and secondary metabolism. Si treatment counteracted these inhibitions to some extent. Specifically, Si treatment increased soluble protein content by approximately 15% by regulating the nitrogen metabolism of G. uralensis under D stress. Furthermore, Si treatment elevated the content of glycyrrhetinic acid by about 89% under SD stress by increasing the content of primary metabolites and regulating the expression of enzymes involved in the biosynthesis of glycyrrhizic acid and liquiritin, including 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), squalene synthase (SQS), and β-amyrin synthase (β-AS). In summary, our findings suggest that Si could alleviate the adverse effects induced by drought and/or salt stresses on the growth of G. uralensis seedlings by regulating nitrogen metabolisms, which further triggered the accumulation of secondary metabolites, ultimately improving the stress resistance of cultivated G. uralensis seedlings. This work provides direction for Si to improve stress resistance.

干旱和盐胁迫下硅对甘草氮和次生代谢的调节作用。
甘草(glycyrhiza uralensis Fisch)是防风固沙的重要植物,具有显著的药理和经济价值。然而,由于其种植在干旱、半干旱和受盐影响的地区,乌拉尔松的产量受到很大影响。据报道,硅(Si)通过调节氮和次生代谢来提高植物对干旱和盐胁迫的耐受性。本研究结合生理和转录组学分析,研究了硅处理对干旱(D)、盐(S)和干旱-盐(SD)胁迫下乌拉尔根(G. uralensis)幼苗氮和次生代谢的影响。结果表明,胁迫条件通过抑制氮代谢和次生代谢显著抑制乌拉尔松幼苗的生长。硅处理在一定程度上抵消了这些抑制作用。具体而言,硅处理通过调节D胁迫下乌拉尔稻的氮代谢,使可溶性蛋白含量提高了约15%。此外,Si处理通过增加主要代谢物的含量和调节参与甘草酸和甘草素生物合成的酶,包括3-羟基-3-甲基戊二酰辅酶a还原酶(HMGR)、角鲨烯合成酶(SQS)和β-amyrin合成酶(β-AS)的表达,使SD胁迫下甘草次酸含量提高了89%左右。综上所述,硅可以通过调节氮代谢,进而引发次生代谢物的积累,从而缓解干旱和/或盐胁迫对乌拉尔根幼苗生长的不利影响,最终提高乌拉尔根幼苗的抗逆性。本研究为硅提高抗逆性提供了方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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