Resolution enhancement of total internal reflection microscopy via polarised scattering.

IF 1.5 4区工程技术 Q3 MICROSCOPY

Journal of microscopy Pub Date : 2025-03-05 DOI:10.1111/jmi.13396

Chenguang Liu, Zhao Chen, Zhenlong Xu, Qinhai Yang, Jian Liu

引用次数: 0

Abstract

This paper presents a novel approach for enhancing the resolution of label-free total internal reflection microscopy. This method leverages the directional difference of the sample polar scattering by capturing microscopic images under the illumination of evanescent waves propagating in various directions. By computing the high-order autocorrelation accumulation of these images at different angles, we achieved a 1.5-fold improvement in resolution while effectively reducing the distortion caused by laser speckles and image artefacts. This technique holds promise for long-term, noninvasive observation with enhanced resolution.

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来源期刊

Journal of microscopy 工程技术-显微镜技术

CiteScore

4.30

自引率

5.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.