Analysis of (-)-11-nor-9-carboxy-Δ9-tetrahydrocannabinol and (-)-11-nor-9-carboxy-Δ8-tetrahydrocannabinol in hair from a school-age population.

IF 2.3 3区 医学 Q3 CHEMISTRY, ANALYTICAL
Ryan B Paulsen, G Neil Stowe, Michael I Schaffer, Nickolaus Krohn
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引用次数: 0

Abstract

A sensitive liquid chromatography-tandem mass spectrometry method for the detection of (-)-11-nor-9-carboxy-Δ9-tetrahydrocannabinol and (-)-11-nor-9-carboxy-Δ8-tetrahydrocannabinol in hair with a cutoff of 1 pg/10 mg of hair and a limit of quantitation of 0.2 pg/10 mg of hair for both analytes was developed and is herein described. A subset of samples collected from a school-age population between December 2022 and February 2023 was analyzed using this method after having screened presumptive positive by enzyme immunoassay out of a total pool of approximately 5300 samples. Of these presumptive positive samples, 66% showed the presence of one or both analytes at a concentration ≥1 pg/10 mg of hair. Of the 213 positive samples, 57% contained more (-)-11-nor-9-carboxy-Δ8-tetrahydrocannabinol than (-)-11-nor-9-carboxy-Δ9-tetrahydrocannabinol and 6% contained more than 50-fold higher Δ8 isomer than Δ9 isomer. Of the 197 samples that were reportable for (-)-11-nor-9-carboxy-Δ9-tetrahydrocannabinol ≥1 pg/10 mg cutoff, 53% of them contained more (-)-11-nor-9-carboxy-Δ8-tetrahydrocannabinol than (-)-11-nor-9-carboxy-Δ9-tetrahydrocannabinol. Of the 197 samples that were reportable for (-)-11-nor-9-carboxy-Δ8-tetrahydrocannabinol ≥1 pg/10 mg cutoff, 15.7% exceeded the upper limit of linearity of the method (200 pg/10 mg). These results suggest a high level of (-)-Δ8-tetrahydrocannabinol usage in this population relative to (-)-Δ9-tetrahydrocannabinol usage. They further suggest the possibility that (-)-11-nor-9-carboxy-Δ9-tetrahydrocannabinol reported for some of these samples may only have been present due to (-)-Δ9-tetrahydrocannabinol contamination of (-)-Δ8-tetrahydrocannabinol products being consumed in large quantities. Thus, reported results for (-)-11-nor-9-carboxy-Δ9-tetrahydrocannabinol alone may give a false picture of the extent of the cannabis product being consumed by a test subject.

学龄人群头发中(-)-11-不-9-羧基-Δ9-tetrahydrocannabinol和(-)-11-不-9-羧基-Δ8-tetrahydrocannabinol的分析。
建立了一种灵敏的液相色谱串联质谱法,用于检测头发中的(-)-11-不-9-羧基-Δ9-tetrahydrocannabinol和(-)-11-不-9-羧基-Δ8-tetrahydrocannabinol,两种分析物的下限为1 pg/10 mg头发,定量限为0.2 pg/10 mg头发。从2022年12月至2023年2月期间从学龄人口中收集的样本子集在通过酶免疫测定从大约5300个样本中筛选推定阳性后,采用这种方法进行了分析。在这些推定阳性样本中,66%显示存在一种或两种浓度≥1pg / 10mg头发的分析物。在213个阳性样品中,57%的样品含有(-)-11-不-9-羧基-Δ8-tetrahydrocannabinol比(-)-11-不-9-羧基-Δ9-tetrahydrocannabinol多,6%的样品含有比Δ9多50倍以上的Δ8异构体。在197个(-)-11-不-9-羧基-Δ9-tetrahydrocannabinol≥1 pg/10 mg截止值的样品中,53%的样品含有(-)-11-不-9-羧基-Δ8-tetrahydrocannabinol多于(-)-11-不-9-羧基-Δ9-tetrahydrocannabinol。197个样品中(-)-11-不-9-羧基-Δ8-tetrahydrocannabinol≥1 pg/10 mg截止,15.7%超过该方法的线性上限(200 pg/10 mg)。这些结果表明,相对于(-)-Δ9-tetrahydrocannabinol的使用,该人群中(-)-Δ8-tetrahydrocannabinol的使用水平较高。他们进一步提出,在这些样品中报告的(-)-11-不-9-羧基-Δ9-tetrahydrocannabinol可能只是由于(-)-Δ9-tetrahydrocannabinol污染了大量食用的(-)-Δ8-tetrahydrocannabinol产品。因此,仅报告(-)-11-不-9-羧基-Δ9-tetrahydrocannabinol的结果可能会对测试对象消耗的大麻产品的程度给出错误的描述。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.10
自引率
20.00%
发文量
92
审稿时长
6-12 weeks
期刊介绍: The Journal of Analytical Toxicology (JAT) is an international toxicology journal devoted to the timely dissemination of scientific communications concerning potentially toxic substances and drug identification, isolation, and quantitation. Since its inception in 1977, the Journal of Analytical Toxicology has striven to present state-of-the-art techniques used in toxicology labs. The peer-review process provided by the distinguished members of the Editorial Advisory Board ensures the high-quality and integrity of articles published in the Journal of Analytical Toxicology. Timely presentation of the latest toxicology developments is ensured through Technical Notes, Case Reports, and Letters to the Editor.
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