The Evolution of Locally Adaptive Chromosome Inversions in Mimulus guttatus.

IF 4.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Leslie M Kollar, Lauren E Stanley, Sunil K Kenchanmane Raju, David B Lowry, Chad E Niederhuth
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引用次数: 0

Abstract

Chromosomal inversion polymorphisms are ubiquitous across the diversity of diploid organisms and play a significant role in the evolution of adaptations in those species. Inversions are thought to operate as supergenes by trapping adaptive alleles at multiple linked loci through the suppression of recombination. While there is now considerable support for the supergene mechanism of inversion evolution, the extent to which inversions trap pre-existing adaptive genetic variation versus accumulate new adaptive variants over time remains unclear. In this study, we report new insights into the evolution of a locally adaptive chromosomal inversion polymorphism (inv_chr8A), which contributes to the adaptive divergence between coastal perennial and inland annual ecotypes of the yellow monkeyflower, Mimulus guttatus. This research was enabled by the sequencing, assembly and annotation of new annual and perennial genomes of M. guttatus using Oxford Nanopore long-read sequencing technology. In addition to the adaptive inv_chr8A inversion, we identified three other large inversion polymorphisms, including a previously unknown large inversion (inv_chr8B) nested within inv_chr8A. Through population genomic analyses, we determined that the nested inv_chr8B inversion is significantly older than the larger chromosomal inversion in which it resides. We also evaluated the potential role of key candidate genes underlying the phenotypic effects of inv_chr8A. These genes are involved in gibberellin biosynthesis and anthocyanin regulation. Although little evidence was found to suggest that inversion breakpoint mutations drive adaptive phenotypic effects, our findings do support the supergene mechanism of adaptation and suggest it may sometimes involve nested inversions that evolve at different times.

麻豆的局部适应性染色体倒位进化。
染色体倒位多态性在二倍体生物的多样性中普遍存在,并在这些物种的适应进化中起着重要作用。逆转录被认为是通过抑制重组,在多个连锁位点捕获自适应等位基因,从而起到超基因的作用。虽然现在对反转进化的超基因机制有相当大的支持,但反转在多大程度上捕获了预先存在的适应性遗传变异,而在多大程度上随着时间的推移积累了新的适应性变异,仍然不清楚。在这项研究中,我们报道了一种局部适应性染色体反转多态性(inv_chr8A)的进化新见解,该多态性有助于沿海多年生和内陆一年生黄猴花生态型之间的适应性差异。本研究利用Oxford Nanopore长读测序技术,对古塔atus一年生和多年生新基因组进行测序、组装和注释。除了自适应的inv_chr8A反转,我们还发现了另外三个大的反转多态性,包括嵌套在inv_chr8A中的一个以前未知的大反转(inv_chr8B)。通过群体基因组分析,我们确定嵌套的inv_chr8B反转比它所在的更大的染色体反转要古老得多。我们还评估了inv_chr8A表型效应背后的关键候选基因的潜在作用。这些基因参与赤霉素的生物合成和花青素的调控。虽然很少有证据表明反转断点突变驱动适应性表型效应,但我们的研究结果确实支持了适应的超基因机制,并表明它有时可能涉及在不同时间进化的嵌套反转。
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来源期刊
Molecular Ecology
Molecular Ecology 生物-进化生物学
CiteScore
8.40
自引率
10.20%
发文量
472
审稿时长
1 months
期刊介绍: Molecular Ecology publishes papers that utilize molecular genetic techniques to address consequential questions in ecology, evolution, behaviour and conservation. Studies may employ neutral markers for inference about ecological and evolutionary processes or examine ecologically important genes and their products directly. We discourage papers that are primarily descriptive and are relevant only to the taxon being studied. Papers reporting on molecular marker development, molecular diagnostics, barcoding, or DNA taxonomy, or technical methods should be re-directed to our sister journal, Molecular Ecology Resources. Likewise, papers with a strongly applied focus should be submitted to Evolutionary Applications. Research areas of interest to Molecular Ecology include: * population structure and phylogeography * reproductive strategies * relatedness and kin selection * sex allocation * population genetic theory * analytical methods development * conservation genetics * speciation genetics * microbial biodiversity * evolutionary dynamics of QTLs * ecological interactions * molecular adaptation and environmental genomics * impact of genetically modified organisms
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