Protein Interaction with Zwitterionic Spherical Polyelectrolyte Brushes as Observed by Small Angle X-ray Scattering.

IF 5.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules Pub Date : 2025-03-04 DOI:10.1021/acs.biomac.4c01782

Ziyu Zhang, Yuhua Zhang, Xin Liu, Jiangtao Guo, Li Li, Weihua Wang, Guofeng He, Xuhong Guo

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Abstract

Understanding how zwitterionic spherical polyelectrolyte brushes (SPB) fulfill their antifouling functions requires knowledge of their interactions with exogenous nanoparticles, such as proteins. In this study, zwitterionic SPB were synthesized by grafting 3-[(2-(methacryloyloxy)ethyl)dimethylammonio]propanoate (CBMA) and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide (SBMA) onto a polystyrene core via photoemulsion polymerization. Small-angle X-ray scattering was employed to elucidate the interactions and protein adsorption behaviors of the zwitterionic SPB and proteins. SAXS results revealed that both PCBMA SPB and PSBMA SPB exhibit minimal protein adsorption compared with cationic and anionic SPB. PSBMA SPB maintained a consistent resistance to protein adsorption across various conditions. However, PCBMA SPB demonstrated tunable protein adsorption properties, enabled by the controllable ionization of carboxyl groups on the brush chains while maintaining consistently low overall adsorption. These insights enhance our understanding of zwitterionic SPB and offer an experimental and theoretical basis for their application in biomaterials and antifouling technologies.

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.