Label-Free Spectrofluorometric Method for Simultaneous Estimation of Methocarbamol and Aspirin in Biological and Pharmaceutical Matrices: Whiteness and Greenness Evaluation

IF 3.2 4区 化学 Q2 CHEMISTRY, ANALYTICAL
Luminescence Pub Date : 2025-03-05 DOI:10.1002/bio.70139
Heba Abd El-Aziz, Abdallah M. Zeid
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引用次数: 0

Abstract

A combination of methocarbamol and aspirin is commonly used for the treatment of acute musculoskeletal pain, such as low back and neck pain. Simultaneous analysis of these analytes in dosage forms and biofluids using rapid, sensitive, and selective methods would be highly beneficial. Herein, we report the first direct, label-free spectrofluorometric method for simultaneously quantifying methocarbamol (MET) and aspirin (ASP) in coformulated tablets and spiked human urine. The method utilizes excitation at 274 nm with independent emission detection at 300 nm for MET and 402 nm for ASP, using methanol as the diluting solvent. The assay was rectilinear over ranges of 0.10–0.80 μg/mL for MET and 0.05–0.50 μg/mL for ASP with detection limits of 14.9 and 10.6 ng/mL for MET and ASP, respectively. The method was validated in compliance with ICH regulations. The approach was applied efficiently to assay both analytes in their tablets and spiked human urine with high % recoveries (98%–103%) and low RSD (1.7%–3.3%). In addition, the sustainability and practicality of the developed method were assessed using the RGB-12 whiteness algorithm, indicating the whiteness of the developed method. The method's greenness was also confirmed using three greenness metrics that showed excellent eco-friendliness of the developed method.

同时测定生物和药物基质中甲氨基酚和阿司匹林的无标记荧光光谱法:白度和绿度评价
甲氨氨基酚和阿司匹林的组合通常用于治疗急性肌肉骨骼疼痛,如腰痛和颈部疼痛。使用快速、灵敏和选择性的方法同时分析剂型和生物流体中的这些分析物将是非常有益的。在此,我们报告了第一个直接的,无标记的荧光光谱法同时定量复方片剂和加标人尿中的甲氨基酚(MET)和阿司匹林(ASP)。该方法使用甲醇作为稀释溶剂,在274 nm处激发,在300 nm处进行MET和402 nm处进行ASP的独立发射检测。在MET 0.10 ~ 0.80 μg/mL和ASP 0.05 ~ 0.50 μg/mL范围内呈线性关系,MET和ASP的检出限分别为14.9和10.6 ng/mL。该方法符合ICH相关规定。该方法可有效地测定其片剂和加标尿液中的两种分析物,回收率高(98% ~ 103%),RSD低(1.7% ~ 3.3%)。此外,采用RGB-12白度算法对所开发方法的可持续性和实用性进行了评估,表明所开发方法的白度。该方法的绿色度也证实了使用三个绿色度指标,显示了良好的生态友好的开发方法。
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来源期刊
Luminescence
Luminescence 生物-生化与分子生物学
CiteScore
5.10
自引率
13.80%
发文量
248
审稿时长
3.5 months
期刊介绍: Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.
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