Investigating the Cytoprotective Effect of Syzygium aromaticum and Elettaria cardamomum in Arsenic-Induced Toxicity in the HCT-116 Cell Model: A Luminesce-Based Study

IF 3.2 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2025-03-05 DOI:10.1002/bio.70119

Vinod Kumar Nelson, Selva Kumar Radha Krishnan, Ayed A. Dera, Syed Parween Ali, M Yasmin Begum, Gowri Sankara Rao Burle, Punna Rao Suryadevara, Geetha Birudala

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Abstract

Arsenic is a highly menacing environmental pollutant that substantially affects the quality of life, even in traces. It develops various diseases through increasing intracellular ROS that interacts with vital organs. Chelation therapy is currently recommended for arsenic toxicity. However, these treatment options are not specific and only target symptoms. Besides, they also show toxic effects and irreversibly damage crucial organs. Hence, there is an urgent need for a potential and safer therapeutic strategy that can prevent arsenic toxicity. Therefore, in this study, we have investigated SA and EC individually and in combination against arsenic toxicity in HCT-116 cells. The results obtained from various experiments revealed that both plant materials comparably inhibited arsenic-induced cellular damage by increasing the antioxidant function. Conversely, combining both plants showed even better cytoprotection against arsenic toxicity. The FT-IR and HPLC analysis of the active fractions revealed the presence of polyphenol compounds (ferulic acid and gallic acid) responsible for the pharmacological effect. This finding is unique because no cytoprotective impact has been reported yet on this combination.

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在HCT-116细胞模型中探讨香薷和豆蔻对砷毒性的细胞保护作用：基于发光的研究

砷是一种极具威胁性的环境污染物，即使微量也会严重影响生活质量。它通过增加与重要器官相互作用的细胞内ROS来发展各种疾病。螯合治疗目前被推荐用于砷中毒。然而，这些治疗方案并不具体，只针对症状。此外，它们还显示出毒性作用，对重要器官造成不可逆转的损害。因此，迫切需要一种潜在的和更安全的治疗策略，可以防止砷中毒。因此，在本研究中，我们分别和联合研究了SA和EC对HCT-116细胞的砷毒性作用。各种实验结果表明，这两种植物材料通过增加抗氧化功能来抑制砷诱导的细胞损伤。相反，两种植物的结合显示出更好的抗砷毒性的细胞保护。红外光谱（FT-IR）和高效液相色谱（HPLC）对活性组分进行分析，发现其药理作用主要由多酚类化合物（阿魏酸和没食子酸）组成。这一发现是独特的，因为目前还没有关于这种组合的细胞保护作用的报道。

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来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.