Yijie Zhou, Qiuxiang Yu, Pengju Dong, Zhusheng Lan, Yize Zhang, Tianwei Li, Guijun Miao, Michael G. Mauk, Yuhuan Fu, Ganli Nie, Xin Sun, Lei Wang, Lulu Zhang, Xianbo Qiu
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引用次数: 0
Abstract
A microfluidic system for immunohistochemistry providing improved staining uniformity and more convenient operation is designed, prototyped, and tested. The chip is comprised of two parts: a plastic (polycarbonate PC) sliding cover that forms a chamber over a glass slide with a mounted sample tissue section. Staining reagents and labeled antibodies are successively pipetted into the chamber and flow over the tissue section by gravity. Staining uniformity is improved in channel design optimization. The plastic cover includes structural features to modify the flow field and reduce the mixing of successive loadings. Flow characteristics are optimized using finite element modeling. The approach shows substantially more uniform staining, as demonstrated quantitatively by image processing of stained samples.
期刊介绍：
Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include:
1.000 Fundamental principles of micro- and nanoscale phenomena like,
flow, mass transport and reactions
3.000 Theoretical models and numerical simulation with experimental and/or analytical proof
4.000 Novel measurement & characterization technologies
5.000 Devices (actuators and sensors)
6.000 New unit-operations for dedicated microfluidic platforms
7.000 Lab-on-a-Chip applications
8.000 Microfabrication technologies and materials
Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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