Characterization of melanin from the fungus Scolecobasidium Musae and its antioxidant and photoprotective properties

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-03-06 DOI:10.1007/s00203-025-04279-2

Fatma Meryem Bozhuyuk, Murat Ozdal

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Abstract

Melanin is a dark colored pigment produced by various living organisms. In this study, the melanin-producing fungus was isolated from a washing machine and identified as Scolecobasidium musae by 18 S rDNA analysis. Melanin production by S. musae was reported for the first time. Melanin was obtained from the dark-colored biomass produced by S. musae in submerged culture. The pigment was then purified, characterized, and identified. It was determined that purified melanin was dissolved easily in alkaline solutions but insoluble in organic solvents. Devices such as UV-Vis spectroscopy, XRD, FTIR, NMR, EPR, and elemental analysis were used to determine the chemical content and structure of the produced melanin. The melanin synthesis pathway was investigated using melanin inhibitors and by performing LC-MS analysis of the fermentation medium in which S. musae grew. It was found that the newly produced melanin from S. musae possesses antioxidant and photoprotective properties (sun protection and UV protection for Staphylococcus aureus). These results provide new possibilities for the biotechnological applications of this naturally producing melanin pigment.

Graphical Abstract

Abstract Image

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Musae真菌黑素的鉴定及其抗氧化和光保护作用

黑色素是一种由各种生物产生的深色色素。本研究从洗衣机中分离得到一种产黑色素真菌，经18s rDNA分析鉴定为musae Scolecobasidium。本文首次报道了S. musae产生黑色素的途径。黑色素是由深层培养的霉菌产生的深色生物量中获得的。然后对色素进行纯化、表征和鉴定。结果表明，纯化后的黑色素易溶于碱性溶液，不溶于有机溶剂。利用紫外可见光谱、XRD、FTIR、NMR、EPR、元素分析等仪器测定所得黑色素的化学成分和结构。利用黑色素抑制剂和LC-MS对霉菌生长的发酵培养基进行分析，研究了黑色素合成途径。研究发现，霉菌新产生的黑色素具有抗氧化和光防护作用（对金黄色葡萄球菌具有防晒和防紫外线作用）。这些结果为这种自然产生的黑色素的生物技术应用提供了新的可能性。图形抽象

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.