{"title":"Chitin-amended media: Improving efficacy of Cordyceps fumosorosea as a control agent of Cydia pomonella","authors":"Nushrat Harun Antara, Dietrich Stephan","doi":"10.1016/j.jip.2025.108296","DOIUrl":null,"url":null,"abstract":"<div><div>The microbial control of insect pests such as the codling moth <em>Cydia pomonella</em> is a major challenge in the apple industry. This study investigates the potential of chitin-amended media to improve the efficacy of the entomopathogenic fungus <em>Cordyceps fumosorosea</em> strain JKI-BI-1496 for control of <em>C. pomonella</em>. Chitin, a key component of insect exoskeletons, colloidal chitin (CC) or chitosan were added in two concentrations to the liquid growth media. Only by the amendment of 0.5 % chitosan significant higher numbers of spores were formed whereas the highest biomass was achieved by amendment of chitin. Spores produced in chitosan had no significant effect on the mortality of <em>C. pomonella</em>. But media containing 1 % colloidal chitin (CC) significantly enhanced the efficacy of <em>C. fumosorosea.</em> To achieve 75 % mortality only 1.6 × 10<sup>7</sup> submerged spores/mL were needed in comparison of over four times higher concentrations in media without CC or chitin. However, while 1 % CC positively impacted spore viability during the freeze-drying formulation process, it had a detrimental effect on the fungus’s resilience under prolonged sunlight exposure. These findings provide valuable insights into optimizing media and formulations to enhance the effectiveness of <em>C. fumosorosea</em> in biological control, particularly by improving spore production and formulation processes.</div></div>","PeriodicalId":16296,"journal":{"name":"Journal of invertebrate pathology","volume":"210 ","pages":"Article 108296"},"PeriodicalIF":3.6000,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of invertebrate pathology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022201125000308","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The microbial control of insect pests such as the codling moth Cydia pomonella is a major challenge in the apple industry. This study investigates the potential of chitin-amended media to improve the efficacy of the entomopathogenic fungus Cordyceps fumosorosea strain JKI-BI-1496 for control of C. pomonella. Chitin, a key component of insect exoskeletons, colloidal chitin (CC) or chitosan were added in two concentrations to the liquid growth media. Only by the amendment of 0.5 % chitosan significant higher numbers of spores were formed whereas the highest biomass was achieved by amendment of chitin. Spores produced in chitosan had no significant effect on the mortality of C. pomonella. But media containing 1 % colloidal chitin (CC) significantly enhanced the efficacy of C. fumosorosea. To achieve 75 % mortality only 1.6 × 107 submerged spores/mL were needed in comparison of over four times higher concentrations in media without CC or chitin. However, while 1 % CC positively impacted spore viability during the freeze-drying formulation process, it had a detrimental effect on the fungus’s resilience under prolonged sunlight exposure. These findings provide valuable insights into optimizing media and formulations to enhance the effectiveness of C. fumosorosea in biological control, particularly by improving spore production and formulation processes.
期刊介绍:
The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates.
The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.