Influence of systemic vitamin D administration and local photobiomodulation on bone repair: Histomorphometric, histochemical, and immunohistochemical study in rat calvaria

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-02-25 DOI:10.1016/j.tice.2025.102814

Carolina dos Santos Santinoni , Wesley Fonseca Novaes , Marcela Lucio Caldeira , Graziela Garrido Mori , Liliane Aparecida Tanus Benatti , Maurício Malheiros Badaró , Juliana Ribeiro de Andrade , Thaís Mageste Duque , Gabriel Leonardo Magrin , César Augusto Magalhães Benfatti , Victor Eduardo de Souza Batista , Edilson Ervolino

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引用次数: 0

Abstract

Objective

The purpose of the present study was to evaluate the influence of systemic vitamin D (Vit D) administration with or without local photobiomodulation (PBM) on bone repair of surgically created critical-size defects (CSD) in rat calvaria.

Material and method

Thirty-two rats were used. A 5 mm DTC was created on the calvaria of each animal. The animals were randomly distributed into four experimental groups (n = 8): Control: no treatment; Vit D: systemic Vit D administration through gavage; PBM: local irradiation with low-intensity laser therapy; Vit D/PBM: systemic Vit D administration through gavage and local irradiation with low-intensity laser therapy. The animals were euthanized 15 days after treatments. It was evaluated newly formed bone area (NFBA), percentage of mature and immature collagen fibers (picrosirius red staining and polarized light), immunohistochemical identification of tartrate-resistant acid phosphatase (TRAP), transforming growth factor (TGF), and osteocalcin (OCN). Data were statistically analyzed (ANOVA, Kruskal-Wallis, p < 0.05).

Results

All experimental groups presented similar statistical results in the evaluated parameters. Some qualitative differences were observed, as follows. Groups PBM, Vit D, and Vit D/ PBM presented a slight amount of newly formed bone. Group Vit D presented a higher amount of mature collagen fibers. Groups Vit D and PBM presented higher immunoexpression of OCN than other experimental groups.

Conclusion

It can be concluded that Vit D and PBM did not significantly increase the amount of bone tissue formed but showed a trend towards enhanced bone maturation in the early healing stages of critical-sized defects surgically created in rat calvaria.

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.