Evaluation of the performance of a dengue NS1 assay during the 2024 epidemic in Brazil

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2025-03-05 DOI:10.1016/j.jviromet.2025.115143

Cristina Mendes de Oliveira , Fernanda Yoshika Takahashi , Rodrigo Salazar Silva , Marina Soika , Juliane Nunes Ribeiro , Gabriela Laginestra Francisco , Vanessa Vidotto Frade de Oliveira , Debora Rodrigues de Sousa , José Fernando de Souza , Annelise C.W. Lopes , José Eduardo Levi

{"title":"Evaluation of the performance of a dengue NS1 assay during the 2024 epidemic in Brazil","authors":"Cristina Mendes de Oliveira , Fernanda Yoshika Takahashi , Rodrigo Salazar Silva , Marina Soika , Juliane Nunes Ribeiro , Gabriela Laginestra Francisco , Vanessa Vidotto Frade de Oliveira , Debora Rodrigues de Sousa , José Fernando de Souza , Annelise C.W. Lopes , José Eduardo Levi","doi":"10.1016/j.jviromet.2025.115143","DOIUrl":null,"url":null,"abstract":"<div><div>Accurate diagnosis of dengue in the acute phase is crucial for proper patient management, reducing mortality rates, and enabling public health authorities to implement effective vector control measures. Our study, conducted during the 2024 dengue epidemic in Brazil, compared the results of a rapid dengue NS1 antigen detection assay with dengue RT-PCR using 300 serum specimens. Ninety-five percent of the patients reported symptoms consistent with dengue infection. We observed substantial agreement between the two tests (kappa = 0.8). The rapid NS1 test demonstrated 87 % sensitivity and 92.7 % specificity, using dengue RT-PCR as the gold standard. Our findings confirm that the rapid NS1 antigen detection test can be reliably used during the acute phase of dengue infection to provide an accurate diagnosis.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"335 ","pages":"Article 115143"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of virological methods","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0166093425000369","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Accurate diagnosis of dengue in the acute phase is crucial for proper patient management, reducing mortality rates, and enabling public health authorities to implement effective vector control measures. Our study, conducted during the 2024 dengue epidemic in Brazil, compared the results of a rapid dengue NS1 antigen detection assay with dengue RT-PCR using 300 serum specimens. Ninety-five percent of the patients reported symptoms consistent with dengue infection. We observed substantial agreement between the two tests (kappa = 0.8). The rapid NS1 test demonstrated 87 % sensitivity and 92.7 % specificity, using dengue RT-PCR as the gold standard. Our findings confirm that the rapid NS1 antigen detection test can be reliably used during the acute phase of dengue infection to provide an accurate diagnosis.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.