Sennoside B inhibits malignant phenotypes of triple-negative breast cancer cells and represses ERK/AKT/STAT5 signaling

IF 2.9 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2025-02-13 DOI:10.1016/j.prp.2025.155842

Li-jun Li, Shan-shan Xie

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引用次数: 0

Abstract

Triple-negative breast cancer (TNBC) is the most lethal subtype of breast cancer. Treatment alternatives for TNBC are very limited and new therapeutic drugs are needed. Sennoside B (SB) is a dianthrone glycoside that has shown antitumor activity in osteosarcoma. However, the role of SB in TNBC remains unclear. This study aims to investigate the role and potential mechanism of SB in TNBC. MTT, colony formation, scratch, and Transwell assays were conducted to examine the malignant behaviors of TNBC cells under different doses of SB treatment. RT-qPCR and western blotting were utilized to detect mRNA or protein levels of molecules. The results revealed that SB treatment dose-dependently restrained TNBC cell proliferation, migration, invasiveness as well as epithelial-mesenchymal transition. Mechanistically, SB suppressed extracellular signal-regulated kinase (ERK), Ak strain transforming protein (AKT), and signal transducer and activator of transcription 5 (STAT5) phosphorylation in TNBC cells. In conclusion, SB treatment impairs the cell aggressiveness of TNBC and blocks ERK/AKT/STAT5 signaling in TNBC cells.

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.