Plant growth-promoting effects and possible mechanisms of a plant endophytic fungus Aureobasidium sp. JRF1

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-01 DOI:10.1016/j.plaphy.2025.109724

Zexuan Jiang , Fangren Peng , Jinping Yu , Qi Li

{"title":"Plant growth-promoting effects and possible mechanisms of a plant endophytic fungus Aureobasidium sp. JRF1","authors":"Zexuan Jiang , Fangren Peng , Jinping Yu , Qi Li","doi":"10.1016/j.plaphy.2025.109724","DOIUrl":null,"url":null,"abstract":"<div><div>Endophytic fungi can establish symbiosis with host plant and promote plant growth in a sustainable way. In this study, a previously-isolated plant growth-promoting endophytic fungus JRF1 was deeply studied. JRF1 could colonize <em>Arabidopsis</em> and tomato seedlings and promote their growth. Through sequencing the internal transcribed spacer (ITS) region and <em>18S rRNA</em> gene, JRF1 was identified as an <em>Aureobasidium</em> sp. strain. Transcriptome analysis indicated that JRF1 treatment up-regulated a majority of genes related to calcium signal and genes annotated as peroxidase and glutathione S- transferase. In addition, treatment with JRF1 activated the Aux/IAA (auxin/indole acetic acid) and cytokinin signaling, while down-regulated genes involved in JA/ETH (jasmonic acid/ethylene) pathways. Split co-culture assay not only demonstrated that JRF1 significantly promoted the <em>Arabidopsis</em> growth by direct contacting the seedlings, but also suggested JRF1 could exhibit positive effects in a non-contact manner. Subsequently, metabolome analysis revealed that JRF1 produced many soluble metabolites which might be responsible for plant growth-promoting, and the releasing volatile organic compounds (VOCs) of JRF1 was also isolated and detected. Finally, we found that both cell-free supernatant (CFS) of JRF1 and its fermentation solution could outstandingly promote the plant growth, suggesting its possible role as a microbial fertilizer. Our results uncovered the interaction mode between JRF1 and host plant, proposing that the combined action of JRF1 with its metabolites resulted in the enhanced plant growth<strong>.</strong></div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109724"},"PeriodicalIF":5.7000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825002529","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Endophytic fungi can establish symbiosis with host plant and promote plant growth in a sustainable way. In this study, a previously-isolated plant growth-promoting endophytic fungus JRF1 was deeply studied. JRF1 could colonize Arabidopsis and tomato seedlings and promote their growth. Through sequencing the internal transcribed spacer (ITS) region and 18S rRNA gene, JRF1 was identified as an Aureobasidium sp. strain. Transcriptome analysis indicated that JRF1 treatment up-regulated a majority of genes related to calcium signal and genes annotated as peroxidase and glutathione S- transferase. In addition, treatment with JRF1 activated the Aux/IAA (auxin/indole acetic acid) and cytokinin signaling, while down-regulated genes involved in JA/ETH (jasmonic acid/ethylene) pathways. Split co-culture assay not only demonstrated that JRF1 significantly promoted the Arabidopsis growth by direct contacting the seedlings, but also suggested JRF1 could exhibit positive effects in a non-contact manner. Subsequently, metabolome analysis revealed that JRF1 produced many soluble metabolites which might be responsible for plant growth-promoting, and the releasing volatile organic compounds (VOCs) of JRF1 was also isolated and detected. Finally, we found that both cell-free supernatant (CFS) of JRF1 and its fermentation solution could outstandingly promote the plant growth, suggesting its possible role as a microbial fertilizer. Our results uncovered the interaction mode between JRF1 and host plant, proposing that the combined action of JRF1 with its metabolites resulted in the enhanced plant growth.

Abstract Image

查看原文本刊更多论文

植物内生真菌Aureobasidium sp. JRF1对植物生长的促进作用及其可能机制

内生真菌可以与寄主植物建立共生关系，促进植物的可持续生长。本研究对此前分离到的植物促生长内生真菌JRF1进行了深入研究。JRF1可定殖拟南芥和番茄幼苗，促进其生长。通过内部转录间隔区（ITS）和18S rRNA基因测序，JRF1被鉴定为金黄色葡萄球菌（Aureobasidium sp.）菌株。转录组分析表明，JRF1处理上调了大部分钙信号相关基因以及标记为过氧化物酶和谷胱甘肽S-转移酶的基因。此外，JRF1激活了Aux/IAA（生长素/吲哚乙酸）和细胞分裂素信号通路，下调了JA/ETH（茉莉酸/乙烯）通路的基因。分离共培养实验表明，JRF1不仅可以通过直接接触的方式显著促进拟南芥的生长，还可以通过非接触的方式发挥积极作用。随后的代谢组学分析表明，JRF1产生了许多可能促进植物生长的可溶性代谢物，并分离检测了JRF1释放的挥发性有机化合物（VOCs）。最后，我们发现JRF1的无细胞上清液（CFS）及其发酵液均能显著促进植物生长，提示其可能具有微生物肥料的作用。我们的研究结果揭示了JRF1与寄主植物的相互作用模式，提出JRF1与其代谢物的共同作用导致了植物生长的增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.