The regulation of UV-B - Triggered ABA signal on isoflavones synthesis in soybean suspension cells

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-02-28 DOI:10.1016/j.plaphy.2025.109728

Mian Wang , Yiting Wang , Chong Xie , Pei Wang , Runqiang Yang

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引用次数: 0

Abstract

Isoflavones are abundant antioxidant components in soybeans. UV-B radiation can induce the biosynthesis of isoflavones in soybean suspension cells, but the underlying molecular mechanism remains unclear. The transcriptome analysis revealed that UV-B radiation influenced the biosynthesis and signal transduction of the phytohormone abscisic acid (ABA) during the biosynthesis of isoflavones in soybean suspension cells. This suggests that ABA may be a key mediator of isoflavones synthesis in soybean suspension cells exposed to UV-B. Therefore, this study investigated the critical role of ABA on isoflavones synthesis in soybean suspension cells exposed to UV-B. The content of endogenous ABA increased significantly by 97% in suspension cells exposed to UV-B. Moreover, the application of exogenous ABA led to a rise in intracellular ABA levels. The elevated intracellular ABA triggered the ABA receptor PYL/PYR, thereby initiating ABA signal transduction. This activation, in turn, up-regulated the expression of seven key enzymes involved in isoflavones synthesis, including phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). Consequently, there was a significant increase of 73% and 49% in the total isoflavones content in UV-B and ABA groups, respectively. Additionally, the ABA synthesis inhibitor fluridone suppressed the synthesis of isoflavones in suspension cells. These findings collectively highlight the pivotal role of ABA, a plant signaling molecule, in response to UV-B radiation, culminating in the enhanced accumulation of isoflavones in soybean suspension cells.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.