Specific extraction of ciprofloxacin from milk by magnetic molecularly imprinted polymers

IF 4.5 3区工程技术 Q1 CHEMISTRY, APPLIED

Reactive & Functional Polymers Pub Date : 2025-02-27 DOI:10.1016/j.reactfunctpolym.2025.106227

Shaolin Deng , Xiaolin Yue , Ping Li , Haoyue Xie , Zehua Huang , Wenyuan Tan

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Abstract

In this study, core-shell magnetic molecularly imprinted polymers (MMIPs) were rationally designed using Fe₃O₄@SiO₂ nanoparticles as carriers for selective recognition and extraction of ciprofloxacin (CIP) in milk matrices. Compared with the reported molecularly imprinted polymers, The synthesized MMIPs demonstrated exceptional magnetic responsiveness enabling rapid solid-liquid separation. Static and dynamic adsorption studies revealed a maximum CIP adsorption capacity of 11.64 mg/g, Scatchard model analysis identified two distinct binding sites: high-affinity and low-affinity sites. Pseudo-second-order kinetics indicating chemisorption-dominated binding. Moreover, selective adsorption experiments confirmed high specificity toward CIP adsorption, with no significant interference from structural analogs. Coupled with HPLC-DAD, the method achieved sensitive detection of CIP with limits of detection (LOD) and limits of quantification (LOQ) values of 0.86 g/L and 2.6 g/L, respectively. In the practical application of commercial samples, the recovery rate was 84.11–99.11 %, and the precision (RSD)was 0.5–1.21 %, demonstrating robustness for CIP enrichment in complex milk products.

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磁性分子印迹聚合物对牛奶中环丙沙星的特异性提取

本研究以Fe3O4@SiO2纳米颗粒为载体，合理设计核-壳磁性分子印迹聚合物（MMIPs），对乳基质中的环丙沙星（CIP）进行选择性识别和提取。与报道的分子印迹聚合物相比，合成的MMIPs表现出优异的磁响应性，能够实现快速的固液分离。静态和动态吸附研究表明，CIP的最大吸附量为11.64 mg/g， Scatchard模型分析确定了两个不同的结合位点：高亲和力和低亲和力位点。准二级动力学表明化学吸附为主的结合。此外，选择性吸附实验证实了CIP吸附的高特异性，没有明显的结构类似物干扰。该方法与HPLC-DAD联用，检测限（LOD）和定量限（LOQ）分别为0.86 g/L和2.6 g/L，实现了CIP的灵敏检测。在商业样品的实际应用中，该方法的回收率为84.11 ~ 99.11%，精密度（RSD）为0.5 ~ 1.21%，对复杂乳制品中CIP的富集具有稳健性。

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来源期刊

Reactive & Functional Polymers 工程技术-高分子科学

CiteScore

8.90

自引率

5.90%

发文量

259

审稿时长

27 days

期刊介绍： Reactive & Functional Polymers provides a forum to disseminate original ideas, concepts and developments in the science and technology of polymers with functional groups, which impart specific chemical reactivity or physical, chemical, structural, biological, and pharmacological functionality. The scope covers organic polymers, acting for instance as reagents, catalysts, templates, ion-exchangers, selective sorbents, chelating or antimicrobial agents, drug carriers, sensors, membranes, and hydrogels. This also includes reactive cross-linkable prepolymers and high-performance thermosetting polymers, natural or degradable polymers, conducting polymers, and porous polymers. Original research articles must contain thorough molecular and material characterization data on synthesis of the above polymers in combination with their applications. Applications include but are not limited to catalysis, water or effluent treatment, separations and recovery, electronics and information storage, energy conversion, encapsulation, or adhesion.