Platelet Content from Acute Myocardial Infarction Patients: Elevated Levels of IL-6 and IL-8 and their impact on Endothelial Nitric Oxide Production

IF 4.9 2区医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS

Atherosclerosis Pub Date : 2025-01-28 DOI:10.1016/j.atherosclerosis.2025.119119

Diego Arauna , Emerson Chandia , Estefanía Nova-Lamperti , Claudia Radojkovic , Eduardo Fuentes , Iván Palomo , Enrique Guzmán-Gutiérrez , Rodrigo Moore-Carrasco , Claudio Aguayo

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Abstract

Background and aims

Platelets and inflammation play crucial roles in the atherothrombotic process of acute myocardial infarction (AMI), particularly in ischemia/reperfusion injury. This study aims to characterize the pro-inflammatory content of the platelet secretome in AMI patients, assess its effect on nitric oxide (NO) production, and correlate these findings with clinical parameters.

Methods

Blood samples from 20 AMI patients and 20 controls were analyzed. Platelets were isolated and stimulated with thrombin, and their secretomes were collected. Endothelial cells were exposed to these secretomes, and NO production was measured. Our results demonstrate a significant reduction in NO production in endothelial cells exposed to the AMI platelet secretome (p < 0.05), suggesting acute endothelial dysfunction.

Results

Elevated levels of interleukin-6 (IL-6) (p = 0.0323) and interleukin-8 (IL-8) (p = 0.0197) were identified in the secretome of AMI patients, correlating with clinical markers of myocardial injury. Specifically, IL-6 positively correlated with CK-MB levels (p = 0.04, Pearson r = 0.53), while IL-8 levels inversely correlated with NO production (p = 0.0141, Pearson r = −0.5652).

Conclusions

These findings underscore the critical role of platelet-derived IL-6 and IL-8 in endothelial dysfunction and myocardial injury in AMI patients. Future studies should explore the interactions between these cytokines in endothelial cells to further elucidate their roles in AMI pathology.

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急性心肌梗死患者血小板含量：IL-6和IL-8水平升高及其对内皮细胞一氧化氮生成的影响

背景和目的血小板和炎症在急性心肌梗死（AMI）的动脉粥样硬化血栓形成过程中起着至关重要的作用，特别是在缺血/再灌注损伤中。本研究旨在表征AMI患者血小板分泌组的促炎成分，评估其对一氧化氮（NO）产生的影响，并将这些发现与临床参数联系起来。方法对20例AMI患者和20例对照组的血液样本进行分析。分离血小板，用凝血酶刺激，收集血小板的分泌组。内皮细胞暴露于这些分泌组，并测量NO的产生。我们的研究结果表明，暴露于AMI血小板分泌组的内皮细胞中NO的产生显著减少(p <；0.05)，提示急性内皮功能障碍。结果AMI患者分泌组白细胞介素-6 （IL-6）、白细胞介素-8 （IL-8）水平升高（p = 0.0197），与心肌损伤的临床指标相关。具体而言，IL-6与CK-MB水平呈正相关（p = 0.04, Pearson r = 0.53），而IL-8水平与NO产生负相关（p = 0.0141, Pearson r = - 0.5652）。结论血小板源性IL-6和IL-8在AMI患者内皮功能障碍和心肌损伤中的重要作用。未来的研究应探索内皮细胞中这些细胞因子之间的相互作用，以进一步阐明它们在AMI病理中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Atherosclerosis 医学-外周血管病

CiteScore

9.80

自引率

3.80%

发文量

1269

审稿时长

36 days

期刊介绍： Atherosclerosis has an open access mirror journal Atherosclerosis: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Atherosclerosis brings together, from all sources, papers concerned with investigation on atherosclerosis, its risk factors and clinical manifestations. Atherosclerosis covers basic and translational, clinical and population research approaches to arterial and vascular biology and disease, as well as their risk factors including: disturbances of lipid and lipoprotein metabolism, diabetes and hypertension, thrombosis, and inflammation. The Editors are interested in original or review papers dealing with the pathogenesis, environmental, genetic and epigenetic basis, diagnosis or treatment of atherosclerosis and related diseases as well as their risk factors.