Constructions and immunogenicity evaluations of two porcine epdemic diarrhea virus-like particle vaccines

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2025-03-04 DOI:10.1016/j.vetmic.2025.110451

Hong Xu , Mengdi Yang , Shiyu Liu , Fangyuan Zheng , YuPeng Li , Yunchuan Li , Chuanhong Wang , Jiali Qian , Yongxiang Zhao , Shanshan Yang , Min Sun , Xu Song , Rongli Guo , Jinzhu Zhou , Baochao Fan , Bin Li

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引用次数: 0

Abstract

Porcine epidemic diarrhea virus (PEDV), a swine enteropathogenic coronavirus, causing acute diarrhea, dehydration, and up to 100 % mortality in neonatal suckling piglets, leading to huge economic losses in the global swine industry. Vaccination remains the most promising and effective way to prevent and control PEDV. In this study, we produced PEDV virus-like particles (VLPs) composed of S, M, and E proteins with a baculovirus expression system and a mammalian expression system. The S, M, and E proteins were effectively expressed and successfully assembled into VLPs. Subsequently, S subunits and commercially inactivated vaccines were selected and compared with two VLPs vaccines for immune efficacy through mouse immunization. The results showed that both VLPs induced higher levels of IgG, IgA, and neutralizing antibody titers, lymphocyte proliferation indexes and T, B cell ratios. Compared with the baculovirus VLPs, the mammalian VLPs exhibited better effects in inducing neutralizing antibodies, lymphocyte proliferations, and IFN-γ. These data indicated that the PEDV VLPs vaccine constructed using the mammalian expression system has better immune efficacy and has the potential to serve as a novel PEDV vaccine.

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.