Exposure to PFOS, PFBS, PFOA and PFBA impairs cell cycle progression in bovine brain (Bos taurus) endothelial cells

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-02-26 DOI:10.1016/j.rvsc.2025.105585

Alice Gonella , Marta Giacomello , Livio Finos , Antonella Peruffo

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引用次数: 0

Abstract

Perfluoroalkylated substances (PFAS) are the large class of synthetic chemicals that persist in the environment and bioaccumulate in different tissues including the brain, inducing blood brain barrier (BBB) disruption. In this study, we assessed cytotoxicity of PFAS in a bovine brain endothelial cell line by exposing the cells to increasing concentrations (0.01, 0.1, 1, 10, and 100 μM) of perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorobutane sulfonic acid (PFBS), perfluorobutanoic acid (PFBA) and perfluoro ([5-methoxy-1,3-dioxolan-4-yl]oxy) acetic acid (C6O4). Cell viability, cell cycle profiling and the apoptotic potential were then analyzed. Cells were categorized by grouping nuclei into G0 + G1, Synthesis (S) and Mitotic phases (M), nuclei showing characteristics of senescence and nuclear fragments. By combining high throughput screening with cell nuclei counting for group, we determined the relationship between the dose-response effect of PFAS and their proliferative potential. Our results showed that PFOS decreased the number of cells in S and M phase. PFBS reduced the number of cells in M phase, decreased the senescence phenotype and increased the number of fragment nuclei. PFOA enhanced the number of cells nuclei in S and M phase. The PFBA enhanced the number of nuclei in G0 + G1, S and M phase. C6O4 did not show significant variation under any of the experimental conditions tested. We did not find significant changes in terms of cell viability assay. This bovine endothelial cell line provides an alternative model for studying the mechanisms involved in the decrease of BBB integrity due to PFAS accumulation in a large mammal with large brain.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.