Exploring the dynamics of postoperative steatosis in the regenerating liver: An animal study

IF 1.4 Q3 SURGERY

Surgery open science Pub Date : 2025-03-01 DOI:10.1016/j.sopen.2025.02.005

Andrea Lund , Katrine Holm Andersen , Kasper Jarlhelt Andersen , Jakob Kirkegård , Jens Randel Nyengaard , Frank Viborg Mortensen

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Abstract

Introduction

The rat model of 70 % partial hepatectomy (PH) is commonly used to investigate liver regeneration processes. The aim of this study was to explore the dynamics of hepatic lipid accumulation and its correlation with the proliferation response during the entire regeneration phase after 70 % PH in rats.

Methods

Sixty-four rats underwent 70 % PH and were randomly divided into eight groups for evaluation on post-operative day (POD) 1 to 8. Hepatocyte volume, relative lipid content, and lipid volume per hepatocyte were assessed by stereological analysis.

Results: Lipid volume per hepatocyte reached its peak on POD 1 and POD 2, with mean values of 2895 μm³ (95 % CI: 1756–4034 μm³) and 3090 μm³ (95 % CI: 2277–3903 μm³), respectively. A marked decline was observed by POD 4, with a mean of 1323 μm³ (95 % CI: 985–1741 μm³), which continued through POD 5, reaching 619 μm³ (95 % CI: 136–1102 μm³). From POD 5 onwards, lipid volume remained consistently low, with no significant differences detected between POD 5 and POD 8.