Effects of selected heavy metals on neuronal differentiation of Asian seabass spinal cord cell line

IF 4.9 3区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Marine pollution bulletin Pub Date : 2025-03-05 DOI:10.1016/j.marpolbul.2025.117784

Sivaraj Mithra , Basheer Madiha Fathima , Seepoo Abdul Majeed , Gani Taju , Sakvai Mohammed Safiullah , Arni Mujthaba Aatif , Sugumar Vimal , Amtuz Zehra , Sahayarajan Martina , Ahmed Hussain Ghousia Nisha , Azeez Sait Sahul Hameed

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引用次数: 0

Abstract

Asian seabass spinal cord cell line (SBSC) can be differentiated into neuron-like phenotypes through induction with neurotrophin-3 (NT-3), all-trans-retinoic acid (ATRA) and Insulin-like growth factor-I human (IGFI). Following differentiation, this cell line could be used as an in vitro models for investigating various aspects of neuronal cell biology. The study of metal cytotoxicity involved the use of Asian seabass, an important marine fish. The present study was to determine the cytotoxicity of eleven heavy metals (Fe³⁺, Co²⁺, Cd²⁺, Cu²⁺, Na⁺, Hg²⁺, Zn²⁺, In³⁺, Al³⁺, Lu³⁺ and Cr⁶⁺) in the SBSC cell line based on MTT, neutral red (NR) uptake assays and ROS production. This study determined the effective concentrations (EC₅₀ and EC₁₀) of eleven metals in SBSC cells. The result of the present study demonstrates that the EC₁₀ concentration of selected metals affects the neuronal differentiation in the SBSC cells.

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重金属对亚洲海鱼脊髓细胞系神经元分化的影响

亚洲海鲈脊髓细胞系（SBSC）可通过神经营养因子-3 （NT-3）、全反式维甲酸（ATRA）和胰岛素样生长因子- 1人（IGFI）诱导分化为神经元样表型。分化后，该细胞系可作为研究神经细胞生物学各方面的体外模型。金属细胞毒性的研究使用了一种重要的海鱼——亚洲海鲈。本研究通过MTT、中性红（NR）摄取测定和活性氧（ROS）产生测定了11种重金属（Fe3+、Co2+、Cd2+、Cu2+、Na+、Hg2+、Zn2+、In3+、Al3+、Lu3+和Cr6+）对SBSC细胞系的细胞毒性。本研究测定了11种金属在SBSC细胞中的有效浓度（EC50和EC10）。本研究结果表明，所选金属的EC10浓度影响SBSC细胞的神经元分化。

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来源期刊

Marine pollution bulletin 环境科学-海洋与淡水生物学

CiteScore

10.20

自引率

15.50%

发文量

1077

审稿时长

68 days

期刊介绍： Marine Pollution Bulletin is concerned with the rational use of maritime and marine resources in estuaries, the seas and oceans, as well as with documenting marine pollution and introducing new forms of measurement and analysis. A wide range of topics are discussed as news, comment, reviews and research reports, not only on effluent disposal and pollution control, but also on the management, economic aspects and protection of the marine environment in general.