Root-specific expression of CsNPF2.3 is involved in modulating fluoride accumulation in tea plant (Camellia sinensis)

Abstract

F is a non-essential but potentially harmful element for plants, especially when present in excess. The tea plant is known for its ability to hyperaccumulate F from the soil and eventually accumulates in the leaves; however, how tea plant transport F to the leaves remains unclear. Here, we found that Se can significantly decrease the transport efficiency of F from root to leaf. Therefore, RNA-Sequencing was performed on tea roots co-treated with selenite and fluoride, and then we isolated a plasma membrane-localized F transporter CsNPF2.3 from tea plant roots and examined its role in transport of F in tea plant. The results showed that CsNPF2.3 exhibited F transport activity when heterologously expressed in yeast. Expression pattern analysis revealed that CsNPF2.3 is expressed in epidermal cells, cortex cells and xylem parenchyma cells in roots. Overexpression of CsNPF2.3 in tea roots significantly increased F content in the root, stem and leaf, and enhanced the transport efficiency of F from root to leaf. Furthermore, in 9 tea cultivars, CsNPF2.3 expression in the root was significantly positively correlated with F content in the leaf and root, and the transport efficiency of F from root to leaf. Altogether, these findings suggest that CsNPF2.3 was involved in uptake and transport of F in tea plant.