DNA aptamers targeting P. aeruginosa RNAP†

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-03-03 DOI:10.1039/d5cc00682a

Hari Timilsina , Dmytro Kompaniiets , Satya Prakash Arya , Rick Mason Postema , Raunak Jahan , Andrew Michael Reynolds , Siddhartha Kalpa Samadhi Thennakoon , Bin Liu , Xiaohong Tan

引用次数: 0

Abstract

We present the first DNA aptamers designed to target the RNA polymerase (RNAP) of Pseudomonas aeruginosa. Utilizing SELEX, we identified and examined aptamers, among which the R2 aptamer demonstrated high specificity and significant binding affinity for RNAP. R2 effectively captured RNAP, making it suitable for protein tandem purification and coating applications. These results have revealed that aptamers are valuable tools for investigating P. aeruginosa RNAP.

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针对铜绿假单胞菌RNAP的DNA适体

我们提出了第一个DNA适体设计针对铜绿假单胞菌的RNA聚合酶（RNAP）。我们利用SELEX对适配体进行了鉴定和检测，其中R2适配体对RNAP具有高特异性和显著的结合亲和力。R2有效捕获RNAP，使其适用于蛋白质串联纯化和涂层应用。这些结果表明适体是研究铜绿假单胞菌RNAP的重要工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.