Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A.

IF 4.5 Q1 MICROBIOLOGY
mLife Pub Date : 2025-02-23 eCollection Date: 2025-02-01 DOI:10.1002/mlf2.12163
Pengju Wu, Mengqi Zhang, Yanlu Kou, Shikuan Liang, Jinfeng Ni, Qihong Huang, Yulong Shen
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引用次数: 0

Abstract

In Sulfolobales cells, transcription of the Ups (UV-inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili-mediated cell aggregation and chromosomal DNA import, respectively. Ups is composed of UpsA, UpsB, UpsE, and UpsF, while Ced is composed of CedA, CedA1, CedA2, and CedB. So far, how DNA is transported by these systems is far from clear. Here, we report three novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A, CedD (SiRe_1715) and CedE (SiRe_2100), paralogs of CedB and CedA, and UpsC (SiRe_1957), a paralog of UpsA/UpsB. We developed a DNA import and export assay method, by which we revealed that CedD, CedE, and UpsC are essential for DNA import, while CedE and UpsC are also involved in DNA export together with CedA1 and Ups. Microscopic analysis revealed that upsC is involved in cell aggregation like other Ups genes. In addition, we found that cedB and cedD co-occur in the Crenarchaeal genomes that lack virB4, an essential component of type IV secretion system. Interestingly, CedB and CedD share homology to different parts of VirB4 N-terminal domain and form stable homo-oligomers in vitro. Collectively, our results indicate that CedD, CedE, and UpsC are integral components of the Ced and Ups systems in Sulfolobales.

岛糖酵母REY15A中Ced和Ups系统新组分的鉴定。
在Sulfolobales细胞中,uv诱导的Sulfolobus毛(Sulfolobus pili)和Crenarchaeal system for DNA交换系统(Ced)基因的转录受到DNA损伤的高度诱导,这两个系统分别在毛介导的细胞聚集和染色体DNA输入中起关键作用。Ups由UpsA、UpsB、UpsE和UpsF组成,Ced由CedA、CedA1、CedA2和CedB组成。到目前为止,DNA是如何通过这些系统运输的还远不清楚。在这里,我们报道了Saccharolobus islandicus REY15A中Ced和Ups系统的三个新组件,CedD (SiRe_1715)和CedE (SiRe_2100), CedB和CedA的类比,以及UpsA/UpsB的类比UpsC (SiRe_1957)。我们开发了一种DNA进出口测定方法,通过该方法我们发现CedD, CedE和UpsC对DNA进口至关重要,而CedE和UpsC也与CedA1和Ups一起参与DNA出口。显微镜分析显示,upsC与其他Ups基因一样参与细胞聚集。此外,我们发现cedB和cedD共同出现在缺乏virB4的Crenarchaeal基因组中,virB4是IV型分泌系统的重要组成部分。有趣的是,CedB和CedD与VirB4 n端结构域的不同部分具有同源性,并在体外形成稳定的同源低聚物。总的来说,我们的研究结果表明,CedD、CedE和UpsC是硫代烃中Ced和Ups系统的组成部分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
2.30
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