HuMenSCs Initiate the Uterus Stromal Decidualization in Mouse.

Current stem cell research & therapy Pub Date : 2025-02-28 DOI:10.2174/011574888X344947250221103039

Yibing Han, Zhaoyi Li, Hongliu Shi, Yang Dong, Tai Ip Chan, Gang Xiao, Yi Zhun Zhu

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Abstract

Introduction: Human menstrual blood stem cells (huMenSCs) appear to be pre-clinically safe but a controlled phase I clinical trial is required to determine safety for clinical applications.

Method: HuMenSCs established from healthy donors were free of bacteria, mycoplasma, chlamydia, and endotoxin. P3 (passage 3) huMenSCs expressed the mesenchymal stem cell markers. P6 huMenSCs were developmental multipotential and could translocated into the uterine subepithelial stroma after intrauterine transplantation. After 10 and 15 passages, the huMenSCs kept normal karyotypes.

Results: Gene expression showed that compared with the human umbilical cords mesenchymal stem cells (huMSCs), the huMenSCs affected the stromal cells more effectively. The huMenSCs possibly enhanced the stromal cell multiplication and "decidualization" process initiated by Igfbp1.

Conclusion: Expression of Igfbp1, Atf3, Ptgs2, Hoxa10, Nr4a1, and Fox A2 were significantly increased in the stromal cells of the huMenSCs transplanted uterine.

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HuMenSCs启动小鼠子宫间质脱细胞化。

人经血干细胞（huMenSCs）似乎是临床前安全的，但需要一项对照I期临床试验来确定临床应用的安全性。方法：健康供体培养的人造血干细胞无细菌、支原体、衣原体和内毒素。P3（通道3）huMenSCs表达间充质干细胞标志物。P6 huMenSCs具有发育多能性，可在宫内移植后转入子宫上皮下基质。10、15代后，huMenSCs保持正常核型。结果：与人脐带间充质干细胞相比，人脐带间充质干细胞对基质细胞的影响更大。huMenSCs可能增强了Igfbp1启动的基质细胞增殖和“去个体化”过程。结论：huMenSCs移植子宫间质细胞中Igfbp1、Atf3、Ptgs2、Hoxa10、Nr4a1、Fox A2的表达显著升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Current stem cell research & therapy

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