sqt-3(sc63) is an alternative CRISPR/Cas9 co-conversion marker in Caenorhabditis elegans.

Abstract

The identification of genome-edited individuals by CRISPR/Cas9 in Caenorhabditis elegans often relies on the introduction of a second mutation with a visible phenotype. Popular targets for such co-conversion are dpy-10 and sqt-1 , both located on chromosome II. In this study, we introduce sqt-3 ( sc63 ) V as an alternative CRISPR/Cas9 co-conversion marker to facilitate the generation and detection of genome edits in C. elegans . Its location on chromosome V makes sqt-3 a desirable alternative to dpy-10 and sqt-1 when editing targets on chromosome II. Additionally, the conserved nature of sqt-3 provides a potential applicability in other nematode species.