Corneal Resistance to Enzymatic Digestion After Rose Bengal and Combined Rose Bengal/Riboflavin Cross-Linking Is Oxygen Independent.

IF 2.6 3区医学 Q2 OPHTHALMOLOGY

Translational Vision Science & Technology Pub Date : 2025-03-03 DOI:10.1167/tvst.14.3.1

M Enes Aydemir, Nikki L Hafezi, Nan-Ji Lu, Emilio A Torres-Netto, Mark Hillen, Carina Koppen, Farhad Hafezi

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引用次数: 0

Abstract

Purpose: To assess corneal resistance to enzymatic digestion after rose bengal (RB)/green light and RB/green light followed by riboflavin (RF)/ultraviolet A (UV-A) cross-linking (CXL), with or without oxygen.

Methods: Ex vivo porcine corneal buttons (n = 144) underwent CXL with RB/green or RB/green-RF/UV-A under atmospheric 21% oxygen conditions or in a nitrogen chamber with 0.1% oxygen (hypoxic conditions) to test 10- and 15-J/cm2 fluences. After CXL, corneas were digested with 0.3% collagenase A, and mean digestion times (MDTs) were recorded.

Results: For the non-irradiated control group, the MDT was 19.75 ± 1.34 hours. Under atmospheric oxygen conditions, RB/green CXL yielded MDTs of 33.69 ± 1.4 and 34.38 ± 1.31 hours with fluences of 10 and 15 J/cm2, respectively. RB/green + RF/UV-A showed MDTs of 39.56 ± 1.93 and 51.94 ± 4.2 hours for combined fluences of 10 + 10 J/cm2 and 15 + 15 J/cm2, respectively. Hypoxic RB/green MDTs were 33.88 ± 1.02 and 34.06 ± 1.57 hours, and RB/green + RF/UV-A MDTs were 39.62 ± 2.5 and 50.35 ± 1.59 hours for the same respective fluences. No significant differences were observed between the control groups and corresponding intervention groups (all P > 0.05).

Conclusions: CXL via RB/green and RB/green-RF/UV-A significantly enhanced corneal collagenase digestion resistance, irrespective of oxygen presence. These findings could help optimize infectious keratitis therapy CXL protocols.

Translational relevance: Our findings aid the understanding of the molecular mechanisms underlying the therapeutic effect of CXL and may contribute to refining accelerated PACK-CXL protocols and other CXL treatment strategies.

查看原文本刊更多论文

红玫瑰和联合红玫瑰/核黄素交联后角膜对酶消化的抗性是不依赖于氧的。

目的：评价玫瑰红(RB)/绿光和RB/绿光后核黄素(RF)/紫外线A （UV-A）交联（CXL）在有氧或无氧条件下角膜对酶消化的抵抗力。方法：144只离体猪角膜钮扣在21%氧气条件下或0.1%氧气条件下（缺氧条件下）用RB/green或RB/green- rf /UV-A进行CXL，测试10和15 j /cm2的影响。CXL后，用0.3%胶原酶A消化角膜，记录平均消化时间（MDTs）。结果：未照射对照组MDT为19.75±1.34 h。在常压氧气条件下，RB/绿色CXL的MDTs分别为33.69±1.4和34.38±1.31小时，影响分别为10和15 J/cm2。RB/green + RF/UV-A的MDTs分别为39.56±1.93和51.94±4.2 h，综合影响分别为10 + 10 J/cm2和15 + 15 J/cm2。在相同的影响下，RB/green MDTs分别为33.88±1.02和34.06±1.57小时，RB/green + RF/UV-A MDTs分别为39.62±2.5和50.35±1.59小时。对照组与相应干预组间差异无统计学意义（P < 0.05）。结论：无论是否存在氧气，经RB/green和RB/green- rf /UV-A的CXL均可显著增强角膜胶原酶消化阻力。这些发现有助于优化感染性角膜炎的CXL治疗方案。翻译相关性：我们的研究结果有助于理解CXL治疗效果的分子机制，并可能有助于改进加速PACK-CXL方案和其他CXL治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational Vision Science & Technology Engineering-Biomedical Engineering

CiteScore

5.70

自引率

3.30%

发文量

346

审稿时长

25 weeks

期刊介绍： Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.