Comparative transcriptomics of susceptible and resistant Cucumis metuliferus upon Meloidogyne incognita infection.

IF 3.6 3区生物学 Q1 PLANT SCIENCES

Planta Pub Date : 2025-03-03 DOI:10.1007/s00425-025-04649-6

Xiaoxiao Xie, Junru Lu, Runmao Lin, Jian Ling, Zhenchuan Mao, Jianlong Zhao, Qihong Yang, Shijie Zheng, Yan Li, Richard G F Visser, Yuling Bai, Bingyan Xie

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引用次数: 0

Abstract

Main conclusion: Comparative transcriptomics has identified several candidate genes contributing to the resistance of Cucumis metuliferus against Meloidogyne incognita. The Southern root-knot nematode (Meloidogyne incognita) is a significant threat to Cucurbitaceae crops. The African horned melon (Cucumis metuliferus), a wild relative, exhibits high resistance to this nematode. To explore the resistance mechanism, phenotypic analyses were conducted on a susceptible inbred line (CM27) and a resistant inbred line (CM3). CM3 exhibited enhanced root biomass and significantly higher resistance compared to CM27, with poor nematode development observed in CM3 roots. Transcriptomic profiling at multiple post-infection time points revealed 2243 and 3700 differentially expressed genes (DEGs) in CM3 and CM27, respectively. Among these, the top ten DEGs upregulated exclusively in CM3 were functionally analyzed using virus-induced gene silencing (VIGS). Silencing of EVM0019904 or EVM0017058 in CM3 led to susceptibility to M. incognita. These findings provide novel insights into the resistance mechanisms of M. incognita in C. metuliferus and offer potential resources for breeding nematode-resistant Cucurbitaceae crops.

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来源期刊

Planta 生物-植物科学

CiteScore

7.20

自引率

2.30%

发文量

217

审稿时长

2.3 months

期刊介绍： Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.