[GPSM2 is highly expressed in gastric cancer to affect patient prognosis by promoting tumor cell proliferation].

Q3 Medicine

南方医科大学学报杂志 Pub Date : 2025-02-20 DOI:10.12122/j.issn.1673-4254.2025.02.03

Xue Song, Yue Chen, Min Zhang, Nuo Zhang, Lugen Zuo, Jing Li, Zhijun Geng, Xiaofeng Zhang, Yueyue Wang, Lian Wang, Jianguo Hu

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引用次数: 0

Abstract

Objectives: To explore the association between GPSM2 expression level and gastric cancer progression and analyze the functional pathways and action mechanism of GPSM2.

Methods: We analyzed GPSM2 expression levels in gastric cancer tumors based on data from the GEPIA database and the clinical data of 109 patients. Public databases enrichment analysis were used to assess the impact of GPSM2 expression level on survival outcomes and the functional pathways and action mechanism of GPSM2. We further observed the effects of GPSM2 knockdown and overexpression on proliferation, migration and apoptosis of MGC803 cells using CCK-8 assay, colony formation assay, flow cytometry and immunoblotting and on the growth of MGC803 cell xenografts in nude mice.

Results: Bioinformatic analysis and immunohistochemical staining of the clinical specimens both revealed high GPSM2 expressions in gastric cancer (P<0.01). A high GPSM2 expression was significantly correlated with T3-4 stages, N2-3 stages, a carcinoembryonic antigen (CEA) level ≥5 μg/L, and a carbohydrate antigen (CA) 19-9 level ≥37 kU/L (P<0.05). Cox regression analysis identified high GPSM2 expression as an independent risk factor affecting 5-year survival of the patients (P<0.05). Gene ontology (GO) analysis suggested that GPSM2 was involved in cell cycle regulation. In MGC803 cells, GPSM2 overexpression significantly promoted cell proliferation and G1/S transition and xenograft growth in nude mice. KEGG pathway enrichment analysis indicated that GPSM2 executed its biological functions by regulating the p53 signaling pathway, which was confirmed by the results of immunoblotting experiments showing suppression of p53 signaling pathway activity in GPSM2-over expressing MGC803 cells.

Conclusions: GPSM2 is highly expressed in gastric cancer to affect patient prognosis by promoting tumor cell proliferation and G1/S transition possibly via inhibiting the p53 pathway.

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[GPSM2在胃癌中高表达，通过促进肿瘤细胞增殖影响患者预后]。

目的探讨GPSM2表达水平与胃癌进展的关系，分析GPSM2的功能通路和作用机制：方法：基于GEPIA数据库的数据和109例患者的临床数据，分析GPSM2在胃癌肿瘤中的表达水平。利用公共数据库富集分析评估了GPSM2表达水平对生存结果的影响以及GPSM2的功能通路和作用机制。我们使用CCK-8检测法、集落形成检测法、流式细胞术和免疫印迹法进一步观察了GPSM2敲除和过表达对MGC803细胞增殖、迁移和凋亡的影响，以及对MGC803细胞异种移植裸鼠生长的影响：结果：生物信息学分析和临床标本的免疫组化染色均显示 GPSM2 在胃癌中高表达（PPP 结论：GPSM2 在胃癌中高表达：GPSM2在胃癌中高表达，可能通过抑制p53通路促进肿瘤细胞增殖和G1/S转化，从而影响患者预后。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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