Adipose Tissue-Derived Exosome Maintains Metabolic Balance of Extracellular Matrix in Rat Nucleus Pulposus Cells.

IF 6.6 2区医学 Q1 NANOSCIENCE & NANOTECHNOLOGY

International Journal of Nanomedicine Pub Date : 2025-02-24 eCollection Date: 2025-01-01 DOI:10.2147/IJN.S504649

Ruoyu Zhao, Lei Ma, Joan Li, Sen Liu, Dalong Yang, Guobin Liu, Sidong Yang

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引用次数: 0

Abstract

Purpose: This study aimed to investigate the protective effect of adipose tissue-derived exosomes (AT-Exo) on rat nucleus pulposus cells (NPCs).

Methods: Ultracentrifugation was used to extract exosomes from rat adipose tissue. Transmission electron microscopy (TEM), Western blot, and nanoparticle tracking analysis (NTA) were used to characterize the exosomes. Tert-butyl hydrogen peroxide (TBHP) was used to induce apoptosis of rat NPCs. Cell viability was determined by CCK-8 assay. AT-Exo was administered to investigate its effect on rat NPCs using Western blot and immunofluorescence staining.

Results: AT-Exo was successfully extracted and characterized by NTA, TEM, and Western blots. Uptake assay showed that AT-Exo can be taken up by the NPCs. TBHP (60 μM) resulted in decreased cell viability and increased apoptosis of NPCs. Interestingly, AT-Exo protected NPCs against TBHP, indicated by increased cell viability, decreased apoptosis, upregulated Aggrecan and type II collagen deposition, and downregulated matrix metalloproteinase 3/13.

Conclusion: In summary, rat adipose tissue-derived exosomes can increase the levels of Aggrecan, type II collagen, and Bcl2, and decrease the levels of matrix metalloproteinase 3/13, cleaved caspase3, and Bax. Therefore, rat adipose tissue-derived exosomes can maintain metabolic balance of extracellular matrix and protect against apoptosis in rat nucleus pulposus cells.

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脂肪组织衍生的外泌体维持大鼠核浆细胞细胞外基质的代谢平衡

目的：探讨脂肪组织源性外泌体（AT-Exo）对大鼠髓核细胞（npc）的保护作用。方法：采用超离心法提取大鼠脂肪组织外泌体。透射电子显微镜（TEM）， Western blot和纳米颗粒跟踪分析（NTA）用于表征外泌体。用过氧化叔丁基（TBHP）诱导大鼠NPCs细胞凋亡。CCK-8法测定细胞活力。采用Western blot和免疫荧光染色法观察AT-Exo对大鼠npc的影响。结果：成功提取了AT-Exo，并通过NTA、TEM和Western blots对其进行了表征。摄取实验表明AT-Exo可被npc摄取。thbhp （60 μM）导致NPCs细胞活力下降，凋亡增加。有趣的是，AT-Exo可以保护npc免受thbhp的影响，表现为细胞活力增加，细胞凋亡减少，聚集蛋白和II型胶原沉积上调，基质金属蛋白酶3/13下调。结论：综上所述，大鼠脂肪组织源性外泌体可提高Aggrecan、II型胶原和Bcl2的水平，降低基质金属蛋白酶3/13、cleaved caspase3和Bax的水平。因此，大鼠脂肪组织源性外泌体可以维持大鼠髓核细胞细胞外基质代谢平衡，防止细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Nanomedicine NANOSCIENCE & NANOTECHNOLOGY-PHARMACOLOGY & PHARMACY

CiteScore

14.40

自引率

3.80%

发文量

511

审稿时长

1.4 months

期刊介绍： The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area. With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field. Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.