Delivery of Cavitation Therapy With a Modified Clinical Scanner: In Vitro Evaluation

Abstract

In this study, we design and implement pulses [1.67 MHz, 20–1000 cycles, 0.8–2.5 MPa, and 5–100 ms pulse repetition time (PRT)] suitable for microbubble cavitation treatments with a phased array of a clinical ultrasound scanner. A range of acoustic parameters was evaluated in a tissue-mimicking phantom with suspended Sonazoid microbubbles. Hydrophone measurements were used to optimize the transmit beamforming. A passive cavitation detection (PCD) system was designed to measure the microbubble scattered signals over a 1 s exposure. Postprocessing of the scattered signals evaluated frequency content to extract broadband energy and calculate the inertial cavitation dose (ICD). ICD was maximized at 1000 cycles (maximum pulse length), 5 ms (fastest firing rate), and 2.5 MPa peak negative pressure (PNP) (maximum pressure). Inertial cavitation was only sustained for about three pulses (out of hundreds fired) occurring within the first 100 ms of treatment. Temporal analysis of the first 1000-cycle pulse revealed that broadband energy is sustained for the entire pulse. We also demonstrate that while inertial cavitation is possible with clinically available pulse wave Doppler settings, ICD can be significantly increased using the new conditions suggested in this work. We have delivered successful image-guided cavitation treatment after modifying a clinical scanner and monitored the cavitation dose with a PCD system on a gel phantom with suspended microbubbles. We plan to apply this technique in vivo in animal tumor models next. This work demonstrates the first implementation of long, high-pressure pulses on a clinical scanner that users can optimize for cavitation treatments.