Dianmo Ni, Xiaoqiao Hong, Dingyi Liu, Xueyuan Li, Li Li, Wenwu Liu, Zhaogang Sun, Gang Liu
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引用次数: 0
Abstract
Mycobacterium tuberculosis (Mtb) utilizes a heterodimeric ABC transporter (IrtAB) to extract Fe3+ ions from host cells. This study demonstrates that ultrasensitive fluorescent probes, achieved through the conjugation of fluorophores with the ligand of IrtAB, N14G and N14G-Fe can detect Mycobacterium smegmatis at concentrations as low as 1-10 nM within an incubation period of less than 5 min. Furthermore, these probes effectively label Mycobacterium bovis Bacille Calmette-Guérin BCG and the wild-type Mtb strain H37Rv at a concentration of 0.1 μM after 10 min of incubation, achieving a limit of detection of 34 Colony-Forming Unit for the wild-type Mtb strain H37Rv. Both N14G and N14G-Fe successfully identified Mtb in sputum samples from patients diagnosed with tuberculosis, exhibiting exceptional fluorescence.
期刊介绍：
ACS Central Science publishes significant primary reports on research in chemistry and allied fields where chemical approaches are pivotal. As the first fully open-access journal by the American Chemical Society, it covers compelling and important contributions to the broad chemistry and scientific community. "Central science," a term popularized nearly 40 years ago, emphasizes chemistry's central role in connecting physical and life sciences, and fundamental sciences with applied disciplines like medicine and engineering. The journal focuses on exceptional quality articles, addressing advances in fundamental chemistry and interdisciplinary research.
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