Impact of INSR (rs2229429) G>A genetic polymorphism on response to exogenous insulin in type 1 diabetic Iraqi patients.

Abstract

Objective: Aim: To examine prevalence of genotypic distribution, particularly assessing how genetic polymorphisms in Insulin Receptor gene influence effectiveness of insulin therapy in a sample of Iraqi population.

Patients and methods: Materials and Methods: Effect of Single Nucleotide Polymorphisms rs2229429 G>A have been investigated in 99 T1DM individuals, with a mean age of 12.3 years. These patients were managed with exogenous insulin through a basal-bolus monotherapy regimen. Genotyping was performed using an allele-specific polymerase chain reaction technique, and the data were statistically analyzed.

Results: Results: The prevalence of the minor allele frequency is 12% in a sample of Iraqi population. Homozygous mutant carriers of rs2229429 G>A were 10.479 times at higher risk for developing poor glycemic control (HbA1c >86 mmol/mol) compared to wild genotype in type 1 diabetes mellitus, p=0.008. Ultimately poor responders to exogenous insulin, demonstrating significantly higher plasma insulin receptors levels p<0.001.

Conclusion: Conclusions: The investigated Single Nucleotide Polymorphisms is significantly associated with hyperglycemia in type 1 diabetes mellitus and contributes to the development of double diabetes.