Multi-omics integration analysis and association study reveal the potential of ADIPOQ function in gestational diabetes mellitus.

IF 4.6 2区医学 Q1 ENDOCRINOLOGY & METABOLISM

Nutrition & Diabetes Pub Date : 2025-03-01 DOI:10.1038/s41387-025-00356-z

Xiaoying Li, Tianshuang Jia, Yingnan Wu, Yanqing Peng, Yanan Feng, Liping Gong, Shuang Dong, Jiawei Tian, Litao Sun

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引用次数: 0

Abstract

Aim: To investigate the role of ADIPOQ gene in gestational diabetes mellitus (GDM).

Methods: We genotyped single nucleotide polymorphisms (SNPs) rs266729 and rs1501299 within the ADIPOQ gene in a cohort of 1157 pregnant women of north Chinese Han ethnicity. This cohort comprised 560 pregnant women diagnosed with GDM and 597 pregnant women who exhibited normal oral glucose tolerance test at 24-28 weeks' gestation. All participants were recruited from the Department of Obstetrics and Gynecology at the Second Affiliated Hospital of Harbin Medical University. Additionally, we used conventional bioinformatics analysis methods to conduct multi-omics analysis (transcriptome, epigenome, and single-cell level) of ADIPOQ-regulated GDM.

Results: The systolic blood flow velocity/diastolic blood flow velocity (S/D) ratio of the umbilical artery in GDM patients with CC genotype of rs266729 and GG genotype of rs1501299 was higher than control. Single-cell analysis suggested that ADIPOQ was expressed in extravillous trophoblast (EVT), T cell, monocytes, myelocyte, NK cell and syncytiotrophoblast (SCT). Functional enrichment analysis showed ADIPOQ gene was associated with response to nutrient levels, fat cell differentiation.

Conclusion: The findings of our study indicate a correlation between SNPs of ADIPOQ in GDM patients, and ADIPOQ is involved in the transcriptional regulation of GDM.

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目的：研究ADIPOQ基因在妊娠糖尿病（GDM）中的作用：我们对 1157 名中国北方汉族孕妇的 ADIPOQ 基因中的单核苷酸多态性（SNPs）rs266729 和 rs1501299 进行了基因分型。该队列由 560 名确诊为 GDM 的孕妇和 597 名在妊娠 24-28 周时口服葡萄糖耐量试验正常的孕妇组成。所有参与者均来自哈尔滨医科大学附属第二医院妇产科。此外，我们还采用传统的生物信息学分析方法，对ADIPOQ调控的GDM进行了多组学分析（转录组、表观基因组和单细胞水平）：结果：rs266729基因型为CC型和rs1501299基因型为GG型的GDM患者脐动脉收缩期血流速度/舒张期血流速度（S/D）比值高于对照组。单细胞分析表明，ADIPOQ在绒毛外滋养层细胞（EVT）、T细胞、单核细胞、骨髓细胞、NK细胞和合胞滋养层细胞（SCT）中均有表达。功能富集分析表明，ADIPOQ基因与营养水平反应、脂肪细胞分化有关：我们的研究结果表明，GDM 患者 ADIPOQ 的 SNPs 之间存在相关性，ADIPOQ 参与了 GDM 的转录调控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nutrition & Diabetes ENDOCRINOLOGY & METABOLISM-NUTRITION & DIETETICS

CiteScore

9.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Nutrition & Diabetes is a peer-reviewed, online, open access journal bringing to the fore outstanding research in the areas of nutrition and chronic disease, including diabetes, from the molecular to the population level.