Associations between urinary and follicular fluid concentrations of phthalate metabolites and reproductive outcomes in Brazilian women undergoing fertility treatment

IF 3.3 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2025-02-28 DOI:10.1016/j.reprotox.2025.108868

Carla Giovana Basso , Bruno Alves Rocha , Ivana Rippel Hauer , Jonas Carneiro Cruz , Francisco Furtado Filho , Fernando Barbosa Jr , Anderson Joel Martino-Andrade

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引用次数: 0

Abstract

Existing literature indicates that phthalates can be toxic to the ovaries, negatively affecting female reproduction and potentially influencing outcomes in assisted reproductive technology (ART). However, data on associations between urinary and/or follicular fluid phthalate concentrations and ART outcomes in South American women are scarce. Therefore, in this prospective study, we recruited 93 women (n = 119 cycles) undergoing ART at a fertility clinic in Brazil. They provided urine and follicular fluid (FF) samples, from which we measured the concentrations of the 15 phthalate metabolites more frequently found in Brazilian populations. We documented both laboratorial and clinical outcomes, estimating associations using negative binomial regression. Our findings revealed that specific oocyte and embryo development parameters were associated with urinary concentrations of phthalate metabolite. Particularly, number of follicles, maturation, as well as blastulation were negatively associated with Mono-benzyl phthalate (MBzP). Similarly, urinary mono-ethyl phthalate (MEP) was associated with lower rates of maturation, good quality and blastulation. However, some parameters positively associated with mono-(carboxyisooctyl) phthalate (MCiOP), including maturation, good quality, and blastulation. We also observed certain associations between embryo development and FF concentrations of phthalate metabolite, although the magnitude and direction of these associations differed among various metabolites. Overall, our results suggest that urinary and FF concentrations of phthalate metabolite may be linked to altered outcomes in ART cycles. However, further studies are needed to clarify the extent of this impact. Our results support previous literature and is the first to evaluate urinary and FF phthalate metabolites concentrations in South American women undergoing infertility treatment.

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现有文献表明，邻苯二甲酸盐对卵巢有毒性，会对女性生殖产生负面影响，并可能影响辅助生殖技术（ART）的结果。然而，有关南美女性尿液和/或卵泡液中邻苯二甲酸盐浓度与辅助生殖技术结果之间关系的数据却很少。因此，在这项前瞻性研究中，我们招募了 93 名在巴西一家生殖诊所接受 ART 治疗的女性（n = 119 个周期）。她们提供了尿液和卵泡液（FF）样本，我们从中测量了巴西人群中较常见的 15 种邻苯二甲酸酯代谢物的浓度。我们记录了实验室和临床结果，并使用负二项回归法估算了相关性。我们的研究结果表明，特定的卵母细胞和胚胎发育参数与尿液中邻苯二甲酸酯代谢物的浓度有关。特别是，卵泡数量、成熟度和胚泡形成与邻苯二甲酸单苄酯（MBzP）呈负相关。同样，尿液中的邻苯二甲酸单乙酯（MEP）也与卵泡成熟度、卵泡质量和胚泡形成率较低有关。然而，一些参数与邻苯二甲酸单（羧基异辛酯）酯（MCiOP）呈正相关，包括成熟度、优质率和胚泡形成率。我们还观察到胚胎发育与邻苯二甲酸酯代谢物 FF 浓度之间存在一定的关联，尽管不同代谢物之间的关联程度和方向不同。总之，我们的研究结果表明，尿液和绒毛膜中的邻苯二甲酸酯代谢物浓度可能与 ART 周期的结果改变有关。然而，要明确这种影响的程度还需要进一步的研究。我们的研究结果支持以往的文献，也是首次对接受不孕不育治疗的南美女性尿液和FF邻苯二甲酸酯代谢物浓度进行评估。

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来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.